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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,695 results for: Pol

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Showing: 2881 - 2900 of 7695 results

  3. pOCC102

    Plasmid
    #118883
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118883-plasmid-map-sequence-id-232315

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  8. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Publication
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  14. pZB246-T7-T+

    Plasmid
    #250903
    Purpose
    Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone
    Depositor
    Timothy Whitehead
    Publication
    Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.
    Insert
    Thermally Stable T7 RNA Polymerase
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Arginine 31 to Aspartic Acid, Alanine 61 to Argin…
    Promoter
    pT5-lacUV
    Available since
    Feb. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250903-plasmid-map-sequence-id-499452

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  16. pOCC28

    Plasmid
    #118873
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118873-plasmid-map-sequence-id-232344

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  18. KCNK2

    Plasmid
    #73257
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCNK2 (KCNK2 Human)
    Tags
    TEV-His10-FLAG
    Expression
    Insect
    Mutation
    userdefined:Full_Length 1411
    Promoter
    Polyhedrin
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73257-plasmid-map-sequence-id-150197

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  19. pSG5-BMRF1

    Plasmid
    #72631
    Purpose
    Mammalian expression vector for Epstein-Barr virus polymerase accessory protein
    Depositor
    S. Diane Hayward
    Publication
    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMRF1
    Tags
    D22G and S349N
    Expression
    Mammalian
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 2881 - 2900 of 7695 results