We narrowed to 25,689 results for: pET

1. pET-Sfp R4-4 mutant

   Plasmid

   #173491

   Purpose

   Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

   Depositor

   Jun Yin

   Article

   Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

   Insert

   Sfp-R44
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a(+)-GDH

   Plasmid

   #108954

   Purpose

   heterologous expression of GDH of B. megaterium in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Glucose dehydrogenase IV (GDH IV)
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24a-VHH-std

   Plasmid

   #109417

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-LSSmOrange-CNA35

   Plasmid

   #61605

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   LSSmOrange and CNA35
   

   Tags

   His tag

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaM-6

   Plasmid

   #172771

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-6
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK522-FAST-PETase

   Plasmid

   #214754

   Purpose

   plasmid for expression FAST-PETase in Pseudomonas putida

   Depositor

   Hal Alper

   Article

   Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

   Insert

   FAST-PETase
   

   Expression

   Bacterial

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a SpyCatcher-SnoopCatcher

   Plasmid

   #72324

   Purpose

   Bacterial expression of SpyCatcher linked to SnoopCatcher.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SpyCatcher-SnoopCatcher
   

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Mutation

   35 amino acids at the N-term of SpyCatcher have b…

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaMIgG-14

   Plasmid

   #228994

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-14
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaTdT-39

   Plasmid

   #228980

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-39
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-pro-siRNA-V2

    Plasmid

    #111086

    Purpose

    For production of recombinant siRNAs (pro-siRNAs) in E. coli.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28a-hPKM2

    Plasmid

    #44242

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a (+)-hTFIIB

    Plasmid

    #171084

    Purpose

    Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TF2B (GTF2B Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET24a-VHH-mCherry

    Plasmid

    #109421

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a(+)-FGF1-4X

    Plasmid

    #120282

    Purpose

    Produce human recombinant FGF1-4X in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Basic fibroblast growth factor 1 mutant
    

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET_SZ1-RGG-RGG

    Plasmid

    #124942

    Purpose

    Expression of SZ1-RGG-RGG

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-MH6-EsCas13d

    Plasmid

    #108303

    Purpose

    Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    EsCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-His6-Ubc9

    Plasmid

    #133909

    Purpose

    Bacterial expression of His6-Ubc9

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_6xHis-PARP1-CAT

    Plasmid

    #173943

    Purpose

    Bacterial expression of isolated PARP1 CAT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CAT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3993 pET ubcH7

    Plasmid

    #8654

    Depositor

    Peter Howley

    Article

    Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

    Insert

    UbcH7 (UBE2L3 Human)
    

    Expression

    Bacterial

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pET30a-SARS-CoV2-NP

    Plasmid

    #165090

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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