We narrowed to 3,466 results for: ttl

1. pEC3111 (pSpy1C-Plac(3)_ffluc)

   Plasmid

   #218778

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   deletion of mrfp cassette, mutagenesis of the RBS…

   Promoter

   Plac(3)

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPQD0KN0750

   Plasmid

   #185627

   Purpose

   Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   lacZ
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEINO1

   Plasmid

   #179472

   Purpose

   Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation

   Depositor

   Bogumil Karas

   Article

   Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

   Inserts

   ori
   cat
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN-dCas9-CDA1-UGI

   Plasmid

   #196248

   Purpose

   Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL4-RecApro-dCas9-CDA1-UGI-attR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   RecA promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEYFP-C1-hSERT

   Plasmid

   #70103

   Purpose

   mammalian expression of hSERT with N-terminal YFP fusion

   Depositor

   Harald Sitte

   Article

   Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

   Insert

   SERT (SLC6A4 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEG R2-dsRed-L3 #BGV203

   Plasmid

   #48973

   Purpose

   Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   dsRed
   

   Use

   Gateway cloning vector

   Tags

   IRES (strong)

   Expression

   Mutation

   Promoter

   none

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pBEG R2-iBlast-L3 #BGV204

   Plasmid

   #48982

   Purpose

   Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   blasticidin-S deaminase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Expression

   Mutation

   Promoter

   na

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pOCC304

   Plasmid

   #118897

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with TEVprotease and TwinStrep-MB…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEG R2-iHygro-L3 #BGV206

   Plasmid

   #48985

   Purpose

   Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   hygromycin phosphotransferase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Expression

   Mutation

   Promoter

   na

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pBsVchCAST-pyrE

    Plasmid

    #203813

    Purpose

    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ230-RRVQ

    Plasmid

    #200188

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V, E795Q

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ230-RVQ

    Plasmid

    #200187

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V, E795Q

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pECFP-C1-hSERT

    Plasmid

    #70104

    Purpose

    mammalian expression of hSERT with N-terminal CFP fusion

    Depositor

    Harald Sitte

    Article

    Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

    Insert

    SERT (SLC6A4 Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

    Plasmid

    #192273

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC119

    Plasmid

    #118884

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease and mGFP, cl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

    Plasmid

    #191647

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ266

    Plasmid

    #164713

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

    Plasmid

    #191635

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK354

    Plasmid

    #129698

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120010799
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYES2-gRNA-hyg-MCS

    Plasmid

    #107734

    Purpose

    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    X-3 site gRNA expression cassettes
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52p

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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