We narrowed to 16,621 results for: Jun

1. LCV2_mClover3_LacZ

   Plasmid

   #155103

   Purpose

   lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPSV39-dddAI-FLAG

   Plasmid

   #186558

   Purpose

   Broad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

   Plasmid

   #112289

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Human YAP1-2gamma lacking the last 5 Amino acids …

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LCV2_BFP2

   Plasmid

   #155097

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

   Plasmid

   #112288

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   3 Tyrosines (341, 357 and 394 corresponding to ty…

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LCV2_mCherry_LacZ_sgRNA

   Plasmid

   #155108

   Purpose

   lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP SRBC

   Plasmid

   #68399

   Purpose

   Expression of fluorescently tagged SRBC in mammalian cells

   Depositor

   Rob Parton

   Article

   Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

   Insert

   SRBC (Cavin3 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pBOB-N-3HA-GK_v1

   Plasmid

   #134275

   Purpose

   Lentivector encoding 3XHA-tagged GK (isoform 1)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   3X HA

   Expression

   Mammalian

   Mutation

   524 amino acids, isoform 1

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINVHH_H6_His

   Plasmid

   #201719

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_H6 (S )
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS10-riboE-dddAI

    Plasmid

    #186561

    Purpose

    Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pSpac(hy)

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

    Plasmid

    #171100

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Neurog2 Mouse, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

    Plasmid

    #171099

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Ascl1 Mouse, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LCV2_mCherry_Luciferase_sgRNA

    Plasmid

    #155109

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting Luciferase

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    Luciferase_sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LCV2_Luciferase_sgRNA

    Plasmid

    #155094

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting Luciferase

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    Luciferase_sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBOB-N-3HA-GK_v2

    Plasmid

    #134277

    Purpose

    Lentivector encoding 3XHA-tagged GK (isoform 2)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    553 amino acids, isoform 2

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBOB-N-Flag-GK_v1

    Plasmid

    #134270

    Purpose

    Lentivector encoding Flag-tagged GK (isoform 1)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk isoform 1; 524 amino acids

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMBP-parallel1-GK5_v2 (D443N)

    Plasmid

    #134293

    Purpose

    Expresses GK5 (isoform 2)-D443N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 443 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBP-parallel1-GK5_v2 (D280N)

    Plasmid

    #134290

    Purpose

    Expresses GK5 (isoform 2)-D280N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 280 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBP-parallel1-GK5_v2 (D30N)

    Plasmid

    #134289

    Purpose

    Expresses GK5 (isoform 2)-D30N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 30 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA6-C-Flag-GK5_v1

    Plasmid

    #134281

    Purpose

    Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 1; 516 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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