We narrowed to 3,960 results for: LYC

1. pAAV-Ef1a-Con/Fon oG

   Plasmid

   #131778

   Purpose

   INTRSECT 2.0

   Depositor

   Karl Deisseroth

   Article

   Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

   Insert

   rabies glycoprotein - oG
   

   Use

   AAV; Intrsect

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-KRAS4B(G12V)

   Plasmid

   #35633

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   K-Ras4B(G12V) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Glycine 12 to Valine

   Promoter

   PGK

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPObooster

   Plasmid

   #216160

   Purpose

   Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

   Depositor

   Grant Brown

   Article

   Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

   Inserts

   MKT1(30G) (MKT1 Budding Yeast)
   RME1(ins-308A) (RME1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   MKT1 gene containing an aspartic acid to glycine …

   Promoter

   endogenous promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-PGK-ER-KRAS(G12V)

   Plasmid

   #35635

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   K-Ras4B(G12V) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   ER

   Expression

   Mammalian

   Mutation

   Glycine 12 to Valine

   Promoter

   PGK

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. GSK3betaS9A  (1016)

   Plasmid

   #49492

   Purpose

   expresses human Gsk3beta with S9A (constitutively active) mutation

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   GSK3beta S9A (GSK3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S9A (constitutively active)

   Promoter

   CMV

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS SARS-CoV-2 XBB Spike

   Plasmid

   #195287

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 XBB Spike

   Depositor

   Marceline Côté

   Insert

   SARS-CoV-2 XBB Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: T19I, LPPA24-27…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   Feb. 8, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pMis1_1B

    Plasmid

    #192079

    Purpose

    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

    Depositor

    Markus Buchhaupt

    Article

    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

    Inserts

    KanR
    rhaR
    rhaS
    pBBR1 rep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30-ARH3-His-Sumo-HA

    Plasmid

    #111578

    Purpose

    Expresses ARH3 (amino acids 14-363) in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    ARH3 (ADPRS Human)
    

    Use

    Tags

    6xHis-Sumo and HA

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-13

    Promoter

    T7

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.4_CEACAM6-His

    Plasmid

    #149337

    Purpose

    for eukaryotic expression and purification of CEACAM6-His6

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Tags

    IL-2 signal peptide and polyhistidine

    Expression

    Mammalian

    Mutation

    point mutation (GGA to GCA) to replace 'G-g…

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. β₂AR-mCFP

    Plasmid

    #38260

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    β₂AR (ADRB2 Human)
    

    Use

    Tags

    Flag, mCFP, and signal peptide sequence (MKTIIALS…

    Expression

    Mammalian

    Mutation

    diglycine linker (replacing terminator codon)

    Promoter

    CMV

    Available since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSL3718 (pQCascade_Pse)

    Plasmid

    #200899

    Purpose

    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCas7, PseCas8, PseCas6, PseTniQ
    

    Use

    Tags

    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDmAct5C::Cas9-2A-Neo

    Plasmid

    #176679

    Purpose

    Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    D. melanogaster Act5C

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Syn-ATP

    Plasmid

    #51819

    Purpose

    Optical reporter of presynaptic ATP

    Depositor

    Timothy Ryan

    Article

    Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

    Insert

    Chimeric Synaptophysin-mCherry-Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Within WT luciferase gene, changed Threonine 214 …

    Promoter

    CMV

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. eGFP-KIF1A-Rigor

    Plasmid

    #172207

    Purpose

    Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 251 to Alanine

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAaePUb::Cas9-2A-Neo

    Plasmid

    #176680

    Purpose

    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTFG005_Spike_Inducible_3xFlag

    Plasmid

    #191578

    Purpose

    Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    SARS-CoV-2 Spike Protein (S Synthetic)
    

    Use

    Lentiviral

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PGK-KRAS4A(G12V)

    Plasmid

    #35634

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    K-Ras4A(G12V) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Glycine 12 to Valine

    Promoter

    PGK

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only