We narrowed to 23,585 results for: lenti

1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide2

   Plasmid

   #118201

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide3

   Plasmid

   #118200

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide4

   Plasmid

   #118203

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

   Plasmid

   #118202

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

   Plasmid

   #118160

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD

   Plasmid

   #195544

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

   Plasmid

   #195547

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #223173

   Purpose

   Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hfCas13d (N2V8)
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

    Plasmid

    #223174

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

    Plasmid

    #207953

    Purpose

    nuclease inactive control for TRF1FokI WT

    Depositor

    Roger Greenberg

    Article

    Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag, DD, ER, mCherry and FokI (D450A)

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

    Plasmid

    #195548

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180267

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    EGFR sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid

    #207356

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid

    #161925

    Purpose

    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pEF

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180266

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

    Plasmid

    #195549

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

    Plasmid

    #125430

    Purpose

    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1

    Plasmid

    #118169

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge