We narrowed to 58,592 results for: plasmid
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Viral Prep#22222-AAV1PurposeReady-to-use AAV1 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA. CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFP (Cre-dependent)Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAAV-CamKII-ArchT-GFP (PV2527) (AAV1)
Viral Prep#99039-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsGFPAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC101_TIMER
Plasmid#103057Purposelow copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
ExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSFV3-lacZ
Plasmid#92074PurposeThis is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.DepositorInsertlacZ
UseLacz expression plasmid, for run-off transcriptio…Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b
Plasmid#89898PurposeBacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.DepositorInsertCas13b
ExpressionBacterialPromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFlpStop-attB-UAS-2.1-tdTom
Plasmid#88910PurposeattB FlpStop plasmid, for insertion into MiMIC siteDepositorInsertFlpStop
ExpressionInsectPromoter5XUASAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
TagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW-Flag-CtIP
Plasmid#71108Purposemammalian expession of Flag-CtlPDepositorInsertCtlP
UseLentiviralTagsFlagExpressionMammalianMutationPlease see Depositor CommentsPromoterTRE promoter, Tet ONAvailable SinceNov. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
MXS Chaining Kit
Plasmid Kit#1000000064PurposeA collection of standardized building blocks used to assemble plasmids that are optimized for fluorescence imaging or flow cytometry applications.DepositorApplicationCloning and Synthetic BiologyVector TypeMammalian ExpressionCloning TypeRestriction EnzymeAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
piCRg Entry
Plasmid#58904PurposeCas9/gRNA Entry expression plasmidDepositorInserthSpCas9
ExpressionMammalianAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1(S94A)
Plasmid#59145PurposeYAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factorsDepositorAvailable SinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENExpressionMammalianAvailable SinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Simple-gRNA backbone
Plasmid#51306PurposegRNA expression plasmidDepositorTypeEmpty backboneUseCRISPR; Xenopus expressionPromoterT7Available SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEX-SP-YFP-STIM1(D76A)
Plasmid#18859DepositorInsertStromal interaction molecule 1 (STIM1 Human)
TagsYFPExpressionMammalianMutationD76A mutation - changed aspartate 76 to alanine. …Available SinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant
Plasmid#101201PurposeBacterial expression plasmid for SpCas9, REC3 FRET variantDepositorInsertSpCas9 variant C80S/C574S/S701C/S960C
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S701C and S960CPromoterT7Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant
Plasmid#101200PurposeBacterial expression plasmid for SpCas9, REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C and D273CPromoterT7Available SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A
Plasmid#101181PurposeHuman expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationN692A/M694A/H698A/Q926APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A
Plasmid#101182PurposeHuman expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationN692A/Q695A/H698A/Q926APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A
Plasmid#101183PurposeHuman expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationM694A/Q695A/H698A/Q926APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only