We narrowed to 9,797 results for: otos

1. PKC-delta-IRES-hrGFP

   Plasmid

   #73414

   Purpose

   Mammalian expression of mouse PKC-delta and hrGFP

   Depositor

   Raymond Swanson

   Article

   Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

   Insert

   PKC-delta (Prkcd Mouse)
   

   Use

   Tags

   3xFLAG and IRES-hrGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pSUPERIOR.puro-shp53

   Plasmid

   #38035

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

   Insert

   shp53 (TP53 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

3. gCOTS-pyl

   Plasmid

   #92048

   Purpose

   This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Cyanobacteria s. elongatus genome recombination i…

   Tags

   Expression

   Mutation

   Promoter

   LeuP (native S. elongatus promoter) and PrcbL

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-ING5

   Plasmid

   #13290

   Depositor

   Curtis Harris

   Article

   Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

   Insert

   ING5 (ING5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pBS-TRE-mTagBFP2-WPRE

   Plasmid

   #193332

   Purpose

   Expresses mTagBFP2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A XhoI site in the coding sequence is silenced

   Promoter

   TRE

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mPlum-Gamma-Tubulin-17

   Plasmid

   #55971

   Purpose

   Localization: Centrosomes, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. MAC-SARS-CoV-2 Orf7b

   Plasmid

   #158376

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 Orf7b (ORF7b )
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pMGS59(AID-ARF-P2A-Hygromycin)

   Plasmid

   #138174

   Purpose

   An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

   Insert

   AID-ARF-P2A-Hygromycin
   

   Use

   Tags

   AID-ARF clamp degron tag

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a Gx-NL3

   Plasmid

   #169108

   Purpose

   Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MAC-SARS-CoV-2 NSP15

    Plasmid

    #158366

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP15 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. mCerulean3-EB1-C-18

    Plasmid

    #55415

    Purpose

    Localization: MT End Binding Protein, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pRSM12

    Plasmid

    #101643

    Purpose

    Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    meffCFP
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-P1-N-EGFP-C

    Plasmid

    #159436

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-P1-N-miRFP670-C

    Plasmid

    #159438

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    miRFP670
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSM17

    Plasmid

    #101641

    Purpose

    Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    eqFP486
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EYFP-Clathrin-19

    Plasmid

    #56584

    Purpose

    Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. MAC-SARS-CoV-2 NSP7

    Plasmid

    #158358

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP7 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pJL1-EPA-DNNNS-DQNRT

    Plasmid

    #128397

    Purpose

    In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mRFP-dmBACCS2

    Plasmid

    #67630

    Purpose

    Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    mRFP-dmBACCS2
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Drosophila actin gene 5C gene promoter

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Nxph4-6A-HA

    Plasmid

    #128103

    Purpose

    Express HA tagged Nxph4-6A

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mVenus-CENPB-N-22

    Plasmid

    #56325

    Purpose

    Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    CENPB (CENPB Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 1-169 of CENPB (DNA Binding Domain)

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. TRIN-E-shREN

    Plasmid

    #105585

    Purpose

    Dox-inducible mirE shREN(control shRNA)/dsRED expression with Venus marker and Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    control shRNA targeting luciferase
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KAP3-CT

    Plasmid

    #51396

    Purpose

    expression of EGFP-tagged COOH-terminal AA 661–793 KAP3B in mammalian cells

    Depositor

    Trina Schroer

    Article

    Brown et al Traffic. 2005 Dec;6(12):1114-24.

    Insert

    Kinesin-associated protein 3 (KIFAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pCAG-Bmp15

    Plasmid

    #163594

    Purpose

    Expresses BMP-15 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp15 (Bmp15 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TRIN-shMYB.2652

    Plasmid

    #105575

    Purpose

    Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    MYB shRNA #2652
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MAC-GPR78

    Plasmid

    #158382

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    GPR78 (GPR78 Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. MAC-SARS-CoV-2 NSP8

    Plasmid

    #158359

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP8 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. MAC-SARS-CoV-2 NSP10

    Plasmid

    #158361

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP10 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pJH3666

    Plasmid

    #191447

    Purpose

    Pacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP6s
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2s

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FusionRed-PlastinC-10

    Plasmid

    #56130

    Purpose

    Localization: Cytoskeleton, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Plastin (PLS1 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits