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pLPCX mCER-Prx2

Plasmid

#158225

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

Plasmid

#203911

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C2]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

Plasmid

#203910

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C1]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-iGECInano

Plasmid

#186189

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Bacterial

Promoter

AraC

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCYB-TnsA

Plasmid

#17356

Depositor

Nancy Craig

Article

Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

Insert

TnsA (tnsA E. coli transposon Tn7)

Tags

Intein-Chitin binding domain

Expression

Bacterial

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pYZ673

Plasmid

#132953

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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TRPML3-MYC

Plasmid

#18833

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pJH291

Plasmid

#172153

Purpose

Reporter plasmid for pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.2TAG; Plpp pylS; PproK pylT; AraC

Expression

Bacterial

Mutation

sfGFP(N39*, Y151*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120015170

Plasmid

#46006

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120015170 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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DHFR

Plasmid

#64200

Purpose

Expression of E. coli DHFR

Depositor

Vern Schramm

Article

Wang et al J Am Chem Soc. 2014 Jun 11;136(23):8333-

Insert

Dihydrofolate reductase

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pAC1819_pCR8-DR-SMN2-2-DR

Plasmid

#118653

Purpose

Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-SMN2-2-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM49981

Plasmid

#169087

Purpose

MoClo level 0 BvADHα coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvADHα

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Osh3

Plasmid

#58839

Purpose

Expresses GFP-Tagged Osh3 in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

Insert

Osh3p (OSH3 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pAGM921

Plasmid

#169086

Purpose

MoClo level 0 Db5GT coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Db5GT

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSi

Plasmid

#67884

Purpose

Derivative of pSiM24 plant expression vector without the M24 promoter

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pJT103

Plasmid

#25087

Depositor

Christopher Voigt

Article

Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

Insert

PompC-lacZ

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-hphMX4

Plasmid

#26876

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pAG505-H2B-frFAST

Plasmid

#160056

Purpose

Expresses H2B-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

H2B-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010783

Plasmid

#46009

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010783 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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