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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 42,530 results for: Eras

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Showing: 2961 - 2980 of 42530 results

  1. pAN013

    Plasmid
    #220050
    Purpose
    Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.
    Depositor
    Blake Wiedenheft
    Article
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    GFP-stop-fLuc
    Expression
    Mammalian
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220050-plasmid-map-sequence-id-434403

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  2. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  7. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid
    #208626
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 191~307, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208626-plasmid-map-sequence-id-415912

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  8. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

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  9. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

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  10. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid
    #208647
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208647-plasmid-map-sequence-id-418374

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Showing: 2961 - 2980 of 42530 results