We narrowed to 12,789 results for: siae

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pILGFPQ4

Plasmid

#83559

Purpose

Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL7 promoter-yEGFP

Expression

Yeast

Promoter

GAL7

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPQ4

Plasmid

#83559

Purpose

Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL7 promoter-yEGFP

Expression

Yeast

Promoter

GAL7

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Dom34(full length)

Plasmid

#19098

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
Dom34(full length)

Plasmid

#19098

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
iRFP713-FRB-Mss4-Kina

Plasmid

#202744

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP713-FRB-Mss4-Kina

Plasmid

#202744

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Expression

Bacterial

Promoter

trc promoter

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Expression

Bacterial

Promoter

trc promoter

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330)

Plasmid

#87738

Purpose

Bacterial expression of the VSL domain of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-279

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330)

Plasmid

#87738

Purpose

Bacterial expression of the VSL domain of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-279

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPQ3

Plasmid

#83558

Purpose

Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL2 promoter-yEGFP

Expression

Yeast

Promoter

GAL2

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPQ3

Plasmid

#83558

Purpose

Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL2 promoter-yEGFP

Expression

Yeast

Promoter

GAL2

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Dom34+GST

Plasmid

#19099

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag, GST, and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Dom34+GST

Plasmid

#19099

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag, GST, and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pSP571

Plasmid

#139498

Purpose

CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD Cas9 / sgRNA (STL162)

Expression

Yeast

Mutation

WT

Promoter

pGPD

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSP571

Plasmid

#139498

Purpose

CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD Cas9 / sgRNA (STL162)

Expression

Yeast

Mutation

WT

Promoter

pGPD

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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