Addgene

We narrowed to 2,430 results for: gem

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-KASH5-DN

Plasmid

#171492

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Expression

Mutation

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-GST-KASH5-DN

Plasmid

#171493

Purpose

T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

GST

Expression

Mutation

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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CD11b promoter in pGEM3zf(-)

Plasmid

#26168

Depositor

Daniel Tenen

Article

Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

Insert

CD11b promoter (ITGAM Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21

Plasmid

#105519

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

Promoter

T7

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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Geminin-mStrawberry reporter

Plasmid

#158690

Purpose

This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_GEMIN5:1-1508

Plasmid

#211164

Purpose

Mammalian expression of full-length human GEMIN5. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

Gemin5:M1-M1508 (GEMIN5 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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p3523 pGEM HPV-11 E6

Plasmid

#10854

Depositor

Peter Howley

Article

Werness et al Science. 1990 Apr 6. 248(4951):76-9.

Insert

HPV11 E6

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pGEMHE-membrane-mEGFP

Plasmid

#105526

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

GNAI2 (GNAI2 Human)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

fragment encoding the plasma membrane targeting s…

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-GEMSgRNA2

Plasmid

#204768

Purpose

pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

guide RNA targeting GEMS m6A sensor mRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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