Addgene

We narrowed to 9,510 results for: SUB

Showing: 701 - 750 of 9510 results

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pcDNA3-mouse PKA-RIbeta-mEGFP

Plasmid

#45526

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RI beta tagged by mEGFP

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-MyosinIIA-C-18

Plasmid

#55315

Purpose

Localization: Cytoskeleton, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MyosinIIA

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-rat PKA-RIIbeta-mEGFP

Plasmid

#45523

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta tagged by mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PMT-mScarlet

Plasmid

#203779

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCYP03

Plasmid

#212836

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mouse PKA-Calpha-mPAGFP

Plasmid

#45524

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA catalytic subunit alpha tagged by mPAGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-Palladin-C-7

Plasmid

#55318

Purpose

Localization: Cytoskeleton, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Palladin

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDHJS4 AS+

Plasmid

#78242

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 BS-

Plasmid

#78245

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Use

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 BS+

Plasmid

#78244

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Use

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AS-

Plasmid

#78243

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Use

Tags

Expression

Mutation

Promoter

Available since

June 6, 2016

Availability

Academic Institutions and Nonprofits only
mTagBFP2-CytERM-N-17

Plasmid

#55289

Purpose

Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CytERM

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mTagBFP2-MCHR1-N-10

Plasmid

#55313

Purpose

Localization: Cilium, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MCHR1

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBS rat IRS-3

Plasmid

#11361

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-3 (Irs3 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pIA036

Plasmid

#200425

Purpose

amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Insert

LacI

Use

Tags

bsdronPA

Expression

Bacterial

Mutation

Promoter

Ppen

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCYP10

Plasmid

#212843

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCYP13

Plasmid

#212846

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PMT-mCherry

Plasmid

#203781

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PMT-mCherry2

Plasmid

#203783

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJet1.2 HA6-Tag

Plasmid

#117126

Purpose

C-terminal HA-tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

6xHA Tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

6xHA tag

Expression

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJet1.2 Pk6-tag

Plasmid

#117127

Purpose

C-terminal Pk6-tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

6xPK tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

6xPK tag

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDHJS4 AN-

Plasmid

#78247

Purpose

One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Use

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AN+

Plasmid

#78246

Purpose

One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Use

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pOX + JShal

Plasmid

#16147

Depositor

Larry Salkoff

Article

Jegla et al J Neurosci. 1997 Jan 1. 17(1):32-44.

Insert

Polyorchis penicillatus potassium channel alpha subunit (Shal jelly fish)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pPAmCherry-a-tubulin

Plasmid

#31930

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Use

Tags

a-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pHEN2-SG4

Plasmid

#196071

Purpose

Express wild type SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4

Use

Tags

3XFLAG and 6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
His-TEV-EGFP-PRC1

Plasmid

#167540

Purpose

For expression of His6-TEV-EGFP-PRC1 in bacteria.

Depositor

Tarun Kapoor

Article

Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

Insert

PRC1 (PRC1 Human)

Use

Tags

EGFP and His6 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-MannII-N-10

Plasmid

#55309

Purpose

Localization: Mannosidase/Golgi, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MannII

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
His-TEV-SNAP-PRC1

Plasmid

#167541

Purpose

For expression of His6-TEV-SNAP-PRC1 in bacteria.

Depositor

Tarun Kapoor

Article

Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

Insert

PRC1 (PRC1 Human)

Use

Tags

His6 tag and SNAP tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pVimentin-PSmOrange

Plasmid

#31922

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Vimentin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-ZO1-C-14

Plasmid

#55335

Purpose

Localization: Tight Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

ZO1 (TJP1 Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
PMT-mTurquoise2_2

Plasmid

#203776

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mTurquoise2 dimer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKeratin-PSmOrange

Plasmid

#31924

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Keratin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSmOrange-Tubulin

Plasmid

#31919

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Tubulin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-CAF1-C-10

Plasmid

#55277

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CAF1

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMyosin-PSmOrange

Plasmid

#31921

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Myosin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-mouse PKA-Calpha-mEGFP

Plasmid

#45528

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA catalytic subunit alpha tagged by mEGFP

Use

With chicken beta actin promoter and wpre

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJet1.2 Tev-Avi-tag

Plasmid

#117128

Purpose

C-terminal Tev-Avi tag Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev Avi-tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev Avi-Tag

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
prsTagRFP-N1

Plasmid

#31925

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
PMT-mEGFP_2

Plasmid

#203778

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mEGFP dimer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-SSTR3-N-17

Plasmid

#55326

Purpose

Localization: Cilium, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SSTR3

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
KVS1-HA

Plasmid

#113061

Purpose

Expresses worm KVS-1 in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2005 Nov;8(11):1503-9. doi

Insert

K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-Cx43-7

Plasmid

#55288

Purpose

Localization: Gap Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Cx43

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCAGGS-rat PKA-RIIbeta

Plasmid

#45529

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Use

With chicken beta actin promoter and wpre

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
BS.YxiN.Twin1.a1-479.wt

Plasmid

#13527

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN

Use

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

none

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
DGP104- pSP64 Poly(A)700

Plasmid

#70098

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

700bp insert from B. subtilis

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP105- pSP64 Poly(A)900

Plasmid

#70100

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

900bp insert from B. subtillis

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP106- pSP64 Poly(A)1200

Plasmid

#70101

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

1200bp insert from B. subtillis

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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