We narrowed to 24,805 results for: 034
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Plasmid#193034PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.DepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationV18D, F98D, Residues 1-165PromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)
Plasmid#178034PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deletedPromoterT7LacIAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30e-3p
Plasmid#103418PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30e-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30e-3p target (MIR30E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8
Plasmid#111903PurposeFull length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.DepositorInsertsUseTags8xHis, SNAPf-tag, TEV site, and ZZ-tagExpressionInsectMutationOptimised for expression in Sf9 cells.PromoterAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)
Plasmid#169076PurposeInsect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepIIDepositorInsertsUseTagsGGlinker, TEV cleavage site, StrepII and HIS10 Ta…ExpressionBacterial and InsectMutationPromoterPolyhedrin promoterAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)
Plasmid#169077PurposeInsect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepIIDepositorInsertsUseTagsGFP tag, GGlinker, TEV cleavage site. StrepII and…ExpressionBacterial and InsectMutationPromoterPolyhedrin promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLT1-KDR
Plasmid#86034Purposechimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2DepositorUseTagsHA and t-GFPExpressionMammalianMutationPromoterAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDMJ2-SARS-CoV-2-Spike (B.1-10a)
Plasmid#241034PurposeExpresses SARS-CoV-2 spike (B.1-10a) protein for creating pseudotyped virusDepositorInsertSpike (B.1-10a) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationH69G,K113T,K147N,S155R,T478K,E484V,F490R,S494R,K5…PromoterAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GUCY2D gRNA (BRDN0001145533)
Plasmid#76034Purpose3rd generation lentiviral gRNA plasmid targeting human GUCY2DDepositorInsertGUCY2D (GUCY2D Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-A:U-Hairpin
Plasmid#136034PurposeEIF3B (465-552) 3'UTR with the main hairpin mutated to A and U nucleotides only inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30e-5p
Plasmid#103419PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30e-5p target (MIR30E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30e-3p
Plasmid#103418PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30e-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30e-3p target (MIR30E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLT1-KDR
Plasmid#86034Purposechimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2DepositorUseTagsHA and t-GFPExpressionMammalianMutationPromoterAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8
Plasmid#111903PurposeFull length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.DepositorInsertsUseTags8xHis, SNAPf-tag, TEV site, and ZZ-tagExpressionInsectMutationOptimised for expression in Sf9 cells.PromoterAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
GUCY2D gRNA (BRDN0001145533)
Plasmid#76034Purpose3rd generation lentiviral gRNA plasmid targeting human GUCY2DDepositorInsertGUCY2D (GUCY2D Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 MCS IRES Puro
Plasmid#110342Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.DepositorTypeEmpty backboneUseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS ZfHoxb1a
Plasmid#27519DepositorAvailable SinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 humanTRAF6 (1090)
Plasmid#66930PurposeMammalian Expression of TRAF6DepositorAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 MCS IRES G418
Plasmid#110345Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS ZfHoxb3a
Plasmid#27522DepositorAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only