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pInducer-YAP1-S127A/S397A/S94A

Plasmid

#213586

Purpose

Doxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A, S397A, S94A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A/S311A/S51A

Plasmid

#213590

Purpose

Doxycycline inducible expression of TAZ-S89A/S311A/S51A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A, S311A, S51A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD∆cat

Plasmid

#81054

Purpose

bacterial expression of catalytic dead catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057 with histid…

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-E1051K

Plasmid

#116553

Purpose

Lentiviral expression of PIK3CB E1051K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

E1051K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

Plasmid

#131143

Purpose

Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

mNeonGreen::3XFLAG::Crk (Crk Fly)

Use

Cre/Lox; Attb recombination site

Tags

3XFLAG and mNeonGreen

Expression

Insect

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-D1067Y

Plasmid

#116551

Purpose

Lentiviral expression of PIK3CB D1067Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

D1067Y

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-E554K

Plasmid

#116555

Purpose

Lentiviral expression of PIK3CB E554K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

E554K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-L1049R

Plasmid

#116557

Purpose

Lentiviral expression of PIK3CB L1049R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

L1049R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-N553S

Plasmid

#116558

Purpose

Lentiviral expression of PIK3CB N553S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

N553S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-E470K

Plasmid

#116554

Purpose

Lentiviral expression of PIK3CB E470K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

E470K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-K290N

Plasmid

#116556

Purpose

Lentiviral expression of PIK3CB K290N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

K290N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-R321Q

Plasmid

#116559

Purpose

Lentiviral expression of PIK3CB R321Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

R321Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-A1048V

Plasmid

#116550

Purpose

Lentiviral expression of PIK3CB A1048V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

A1048V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5A delta Tail

Plasmid

#166955

Purpose

Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A (1-820aa)

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1d834-1363-Poly(A)

Plasmid

#65560

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1363)

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1d834-1053-Poly(A)

Plasmid

#65561

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1053)

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-Tet1CD (pc2547)

Plasmid

#246922

Purpose

Expresses mCherry-tagged catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to mCherry.

Depositor

Cristina Cardoso

Article

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

aa1365-2057

Promoter

CAG

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-Tet1CDmut (pc2815)

Plasmid

#246923

Purpose

Expresses mCherry-tagged mutated catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to mCherry.

Depositor

Cristina Cardoso

Article

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

aa1365-2057 / H1652Y, D1654A

Promoter

CAG

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFBDMeGmTet1CD (pc2838)

Plasmid

#246924

Purpose

Expresses mCherry-tagged catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

GFP

Expression

Insect

Mutation

aa1365-2057

Promoter

Polyhedrin Promoter

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFBDMchmTet1CD (pc2859)

Plasmid

#246925

Purpose

Expresses mCherry-tagged catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to mCherry.

Depositor

Cristina Cardoso

Article

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

mcherry

Expression

Insect

Mutation

aa 1365-2057

Promoter

Polyhedrin Promoter

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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