We narrowed to 9,087 results for: Pol;

1. mAC-POLR2A donor (Hygro)

   Plasmid

   #124496

   Purpose

   A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

   Depositor

   Masato Kanemaki

   Article

   Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

   Insert

   POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Perox-mTurq2-Apollo-NADP+

   Plasmid

   #193304

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   mTurqoise2, peroxisomal targeting sequence

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

   Plasmid

   #155193

   Purpose

   Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-4 (Thbs4 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AP1muA-SNAP-V5-polyA-Puro HR

   Plasmid

   #229680

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   SNAP, V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3.7-EF-EYFP-YAP1_S94A-PolyA

   Plasmid

   #112286

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Human YAP1-2gamma with Serine 94 to Alanine

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Wild-type AdPol vector

   Plasmid

   #131685

   Purpose

   A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Insert

   Adenovirus DNA polymerase
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-flag-mKdm4d-polyA

   Plasmid

   #61553

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Kdm4d (Kdm4d Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E-U6a-polg guide

   Plasmid

   #107598

   Purpose

   An entry vector with U6a promoter driving polg guide RNA expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   polg guide RNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-117:POLR2A-mEGFP

   Plasmid

   #164500

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

   Depositor

   Allen Institute for Cell Science

   Insert

   POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Renilla luciferase-Pol III

    Plasmid

    #37380

    Depositor

    Norbert Perrimon

    Article

    Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

    Insert

    PolIII-Renilla control reporter (RpIII128 Fly)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA PolIII 128 subunit (RpIII128)

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-Nup37-EGFP-polyA

    Plasmid

    #104562

    Purpose

    Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Nup37 (Nup37 Mouse)
    

    Use

    Tags

    EGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. attB-GFP-Dnmt3b1-Poly(A)

    Plasmid

    #65531

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 1

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

    Plasmid

    #156164

    Purpose

    Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    THBS1 with addition of ER-retention sequence KDEL

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ef1a-DIO-BiPOLES-mCerulean

    Plasmid

    #154949

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1-FHC-POLQ-DM

    Plasmid

    #64880

    Purpose

    lentiviral expression of human POLQ double mutant (in POL and HDL domains)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M and D2330A,Y2331A

    Promoter

    EF1alpha

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS405-LEU2-TEF-KpolDcr1v2

    Plasmid

    #85193

    Purpose

    Integrating plasmid, K. polysporus Dcr1 under TEF promoter

    Depositor

    David Bartel

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    Dcr1
    

    Use

    Integrating, shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mito-mTurq2-Apollo-NADP+

    Plasmid

    #193303

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2 and mitochondrial targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. hSyn-DIO-BiPOLES-mCerulean

    Plasmid

    #154950

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cyto-mTurq2-Apollo-NADP+

    Plasmid

    #193301

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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