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Showing: 281 - 300 of 13677 results

  4. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  6. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

    Plasmid
    #193942
    Purpose
    a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.
    Depositor
    Greg Delgoffe
    Article
    Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122
    Insert
    heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193942-plasmid-map-sequence-id-389943

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  7. pD649-HAsp-TIMD4-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156731
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TIMD4 (TIMD4 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156731-plasmid-map-sequence-id-401535

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  16. pCMV-T7-SpCas9(RRTIMR)-P2A-EGFP (AHK149)

    Plasmid
    #223071
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RRTIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(RRTIMR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(RRTIMR)=D1135R, S1136R, G1218T, E1219I, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223071-plasmid-map-sequence-id-447687

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  17. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

    Plasmid
    #221112
    Purpose
    FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    STE3
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    221112-plasmid-map-sequence-id-466691

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  18. pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

    Plasmid
    #221114
    Purpose
    FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    221114-plasmid-map-sequence-id-442461

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  20. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

    Plasmid
    #184563
    Purpose
    CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.
    Depositor
    Kacper Rogala
    Article
    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:
    Insert
    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    All lysines were mutated to arginines. Codon-opti…
    Promoter
    CMV
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184563-plasmid-map-sequence-id-376975

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Showing: 281 - 300 of 13677 results