We narrowed to 14,176 results for: TIM

1. pRS415-TEF1pr-MFA-NFAPoptim

   Plasmid

   #221090

   Purpose

   FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBbsr2-EBFP2-OptoSTIM1(CRY2clust)

   Plasmid

   #184716

   Purpose

   OptoSTIM1(CRY2clust)

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   OptoSTIM1
   

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

   Plasmid

   #200639

   Purpose

   Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   STIM1 (STIM1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnYC-NvH-CtNot1optim_1676-2193_U

   Plasmid

   #147729

   Purpose

   Bacterial Expression of CtNot1optim_1676-2193

   Depositor

   Elisa Izaurralde

   Article

   Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

   Insert

   CtNot1optim_1676-2193
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM-60-DmAgo1_572-984-optim_K

   Plasmid

   #146728

   Purpose

   Bacterial Expression of DmAgo1_572-984-optim

   Depositor

   Elisa Izaurralde

   Insert

   DmAgo1_572-984-optim (AGO1 Fly)
   

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWT35S-AtTIM21(1-50aa)-mCherry

   Plasmid

   #194047

   Purpose

   Transient expression of AtTIM21-mCherry in plant cell (Mitochondria)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   mCherry
   

   Tags

   AtTIM21 1–50aa

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-PD-1 (BTLA ITIM)-mGFP

   Plasmid

   #180817

   Purpose

   Expression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   PD-1 (BTLA ITIM) (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Mutation

   replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…

   Promoter

   SFFV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-BTLA (PD-1 ITIM)-mGFP

   Plasmid

   #180818

   Purpose

   Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   BTLA (PD-1 ITIM) (BTLA Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Mutation

   replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…

   Promoter

   SFFV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-optimized E6AP F727D

   Plasmid

   #165101

   Purpose

   ecoli optimized expression of a GST fusion of Human E6AP F727D

   Depositor

   Arthur Haas

   Article

   Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

   Insert

   E6AP F727D (UBE3A Human)
   

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-SIRPT1-Latimeria chalumnae

    Plasmid

    #75324

    Purpose

    GST fusion Latimeria chalumnae Sacsin 94-1368

    Depositor

    Kalle Gehring

    Insert

    Sacsin Latimeria chalumnae 94-1368
    

    Tags

    GST-Tag

    Expression

    Bacterial

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUW PuroR-P2A-COX8ASig-Timer

    Plasmid

    #239559

    Purpose

    Expression and mitohcondrial localization of Timer

    Depositor

    Tim Ahfeldt, Joel Blanchard

    Article

    et al iScience 28 (2025)

    Insert

    Timer
    

    Use

    Lentiviral

    Promoter

    UbC

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDae069 (eMutaT7[PmCDA1] with optimized RBS)

    Plasmid

    #187621

    Purpose

    Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    PmCDA1-T7RNAP
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Str-STIM1-NN_ST-SBP-EGFP

    Plasmid

    #65262

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 His-NLRP1 codon-optimized

    Plasmid

    #166833

    Purpose

    Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129628

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    FHL-cpmTq2-Calcium-lifetime-sensor
    

    Tags

    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

    Expression

    Bacterial and Mammalian

    Promoter

    CMV,&Rhamnose

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lck-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129627

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    Lck-cpmTq2-Calcium-lifetime-sensor
    

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-STIM1-NN_ST-SBP-mCherry

    Plasmid

    #65263

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xnls-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129626

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-cpmTq2-Calcium-lifetime-sensor
    

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-TNT-CFP-STIM1-C227W

    Plasmid

    #68403

    Purpose

    Expresses a STIM1 gain-of-function mutant in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    CFP-STIM1 (STIM1 Human)
    

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Mutation

    Changed Cysteine 227 to Tryptophan; CFP was inser…

    Promoter

    CMV

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. mNeongreen-pGBD(PKN1 codon optimized)

    Plasmid

    #129634

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    RhoA binding doamin of PKN1(30 - 100) codon optimized
    

    Expression

    Mammalian

    Promoter

    CMVdel

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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