We narrowed to 18,942 results for: Tec

1. pcDNA3 Ras-LOCKR-S

   Plasmid

   #216496

   Purpose

   FRET-based reporter for Ras activity, untargeted

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBacDual with PA+Histag

   Plasmid

   #84870

   Purpose

   Baculovirus Expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Baculovirus

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b - PA

   Plasmid

   #84863

   Purpose

   bacterial expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Tags

   pelB signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-6xHis-AviTag-ALFAtag-eGFP

   Plasmid

   #195854

   Purpose

   Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   6xHis-AviTag-ALFAtag-eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PX852

   Plasmid

   #62884

   Purpose

   C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJBL715

   Plasmid

   #140385

   Purpose

   Expresses MphR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-MphR-T
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJBL723

   Plasmid

   #140393

   Purpose

   Expresses HucR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-HucR-T
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PX854

   Plasmid

   #62886

   Purpose

   C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa573-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 PM-Ras-LOCKR-S

   Plasmid

   #216498

   Purpose

   FRET-based reporter for Ras activity, plasma membrane localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   PM-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJBL713

    Plasmid

    #140383

    Purpose

    Expresses CtcS protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CtcS-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPLP1H-mCf

    Plasmid

    #229984

    Purpose

    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPLP2H-mCf

    Plasmid

    #229985

    Purpose

    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPLP3H-mCf

    Plasmid

    #229987

    Purpose

    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJBL701

    Plasmid

    #140371

    Purpose

    Expresses TetR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-TetR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETNb-nALFA

    Plasmid

    #159988

    Purpose

    For the protein expression with ALFA tag at N-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 Golgi-Ras-LOCKR-S

    Plasmid

    #216499

    Purpose

    FRET-based reporter for Ras activity, Golgi localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Golgi-Ras-LOCKR-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 ER-Ras-LOCKR-S

    Plasmid

    #216500

    Purpose

    FRET-based reporter for Ras activity, ER localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    ER-Ras-LOCKR-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 Mito-Ras-LOCKR-S

    Plasmid

    #216501

    Purpose

    FRET-based reporter for Ras activity, mitochondria localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Mito-Ras-LOCKR-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV anti-FITC-LaG16-Y186F-T2A-BFP

    Plasmid

    #194931

    Purpose

    LV construct encoding Y186F mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge