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We narrowed to 4,956 results for: codon optimized

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Showing: 281 - 300 of 4956 results

  1. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    114072-plasmid-map-sequence-id-220522

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  2. pCAG-T3-HypaCAS9-pA

    Plasmid
    #131467
    Purpose
    Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized HypaCas9
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131467-plasmid-map-sequence-id-262453

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  3. pCFJ1320

    Plasmid
    #84829
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84829-plasmid-map-sequence-id-163669

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  4. pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

    Plasmid
    #133798
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19
    Insert
    human codon optimized AcrIIA1(T114A/F115A)
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    T114A/F115A
    Promoter
    CMV and T7
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    133798-plasmid-map-sequence-id-268051

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  5. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107943-plasmid-map-sequence-id-207614

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  6. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid
    #140083
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
    Depositor
    Keith Joung
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human codon optimized pCAG BE4max with P2A-EGFP
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A (SpCas9)
    Promoter
    CAG
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    140083-plasmid-map-sequence-id-292705

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  7. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  9. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  10. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid
    #115140
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA3 anti-CRISPR protein
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    115140-plasmid-map-sequence-id-220495

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  11. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid
    #115138
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA2 anti-CRISPR protein
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    115138-plasmid-map-sequence-id-220494

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  15. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    72248-plasmid-map-sequence-id-131868

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  19. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid
    #114091
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a (E174R/S542R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R and S542R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114091-plasmid-map-sequence-id-220538

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  20. CMV ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid
    #85806
    Purpose
    Genetically encoded voltage sensor ArcLight- codon optimized
    Depositor
    Vincent Pieribone
    Publication
    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ArcLightCo-Q239
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ci-VSP contains R217Q mutation; super ecliptic pH…
    Promoter
    CMV
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    85806-plasmid-map-sequence-id-170999

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Showing: 281 - 300 of 4956 results