We narrowed to 5,126 results for: codon optimized

1. pUB_NonoptλHAramp-FLuc100

   Plasmid

   #210579

   Purpose

   Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Nonoptimal lambda HA-Optimal Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   lambda HA

   Expression

   Mammalian

   Promoter

   UBC human

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_SM-FLuc100-pA

   Plasmid

   #210584

   Purpose

   Expresses SM(FLAG)-optimal Firefly luciferase - pA

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-optimal FLuc
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNB812

   Plasmid

   #60160

   Purpose

   Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Promoter

   RNR1pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-5xT7-TtCsm

   Plasmid

   #128572

   Purpose

   For expression of T. thermophilus Csm in E. coli (Csm1-5)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Csm1
   Csm2, Csm3, Csm4, Csm5
   

   Tags

   HRV 3C protease cleavage site and 10xHis tag on C…

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli and Cod…

   Promoter

   T7 promoter, lac operator

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 469_pME_iCre

   Plasmid

   #171792

   Purpose

   pME-Vector with codon improved iCre sequence. Tol2-kit compatible.

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Insert

   codon-optimized iCre sequence
   

   Promoter

   None

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T1-Opt WWP1

   Plasmid

   #179327

   Purpose

   E. coli optimized expression of GST-fusion of human WWP1

   Depositor

   Arthur Haas

   Insert

   WWP1 (WWP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX4T1-WWP1F792D

   Plasmid

   #179328

   Purpose

   E. coli optimized expression of GST-fusion of human WWP1 F792D

   Depositor

   Arthur Haas

   Insert

   WWP1 F792D (WWP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F792D mutation; Codon Optimized for expression in…

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Bxb1

   Plasmid

   #182142

   Purpose

   Expression of human codon-optimized Bxb1

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   human codon optimized Bxb1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSW002-Pc-mTurquoise2

    Plasmid

    #108235

    Purpose

    Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    Pc promoter - mTurquoise2
    

    Expression

    Bacterial

    Mutation

    codon optimized for experession in P. fluorescens

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-mitoTPNOX

    Plasmid

    #87854

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    mitoTPNOX
    

    Tags

    C-terminal FLAG tag with a linker and mitochondri…

    Expression

    Bacterial

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHsp70-Cas9

    Plasmid

    #45945

    Purpose

    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

    Depositor

    Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Mutation

    Silent change from C to A at bp 834 of insert

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPomyces-1

    Plasmid

    #61736

    Purpose

    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    tracrRNA
    crRNA cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ154

    Plasmid

    #69305

    Purpose

    Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9 (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Mutation

    NLS fusion

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+/N-Halo

    Plasmid

    #184417

    Purpose

    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    Halo-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA flag-Pcbp2

    Plasmid

    #184028

    Purpose

    Codon optimized Pcbp2 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Pcbp2 (Pcbp2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+/C-Halo

    Plasmid

    #184418

    Purpose

    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    Halo-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJY3948

    Plasmid

    #113338

    Purpose

    6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

    Depositor

    Suzanne Sandmeyer

    Article

    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

    Insert

    2-pyrone synthase, codon optimized for Y. lipolytica
    

    Tags

    6XHis

    Expression

    Yeast

    Mutation

    Please see depositor comments

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNB784

    Plasmid

    #60163

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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