We narrowed to 9,530 results for: nsf

1. pEA01

   Plasmid

   #170766

   Purpose

   Utilisation of pSAM2 excision tool in Amycolatopsis spp

   Depositor

   Jean-Luc Pernodet

   Article

   Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

   Inserts

   xis, int
   oriT
   pac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trcp

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF713-pEGFP-N1-Hs-FL-METTL20 wt

   Plasmid

   #85114

   Purpose

   transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKGmDUET-NgBR/hCIT

   Plasmid

   #203173

   Purpose

   Expression of human cis-prenyltransferase complex for purification

   Depositor

   William Sessa

   Article

   Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

   Inserts

   NUS1 (NUS1 Human)
   DHDDS (DHDDS Human)
   

   Use

   Tags

   Strep-Tag II-TEV and internally 6HIS tagged

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3omega1_KanR_BastaR

   Plasmid

   #186426

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) (AAV PHP.eB)

   Viral Prep

   #163505-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) (#163505). In addition to the viral particles, you will also receive purified AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) plasmid DNA. AAV vector for strong and rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core sequence has 100% sequence conservation between mouse and human. Developed especially for rapid viral labeling in human and NHP organotypic brain slice culture. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Promoter

   minBetaGlobin

   Tags

   SYFP2

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

6. pDCAF3-IS5

   Plasmid

   #65220

   Purpose

   Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

   Depositor

   Jeffrey Barrick

   Article

   Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

   Inserts

   ndmA
   ndmB
   ndmC
   ndmD
   gst9
   chlR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed start codon from gtg to atg and Changed s…

   Promoter

   BBa_J23100

   Available since

   Jan. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDGB1alpha2_KanR_BastaR_Rb7_GFP

   Plasmid

   #186427

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   Rb7
   eGFP
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK551

   Plasmid

   #72425

   Purpose

   Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase, N-terminal His6 tag (H6AaUctD)

   Depositor

   T. J. Kappock

   Insert

   putative CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK361

   Plasmid

   #72403

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase, N-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJKW 0633

    Plasmid

    #74122

    Purpose

    Photinus pyralis sulfotransferase ST2 - pHis8-4

    Depositor

    Jing-Ke Weng

    Article

    Fallon et al Biochemistry. 2016 May 26.

    Insert

    Photinus pyralis sulfotransferase 2
    

    Use

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJKW 0690

    Plasmid

    #74123

    Purpose

    Photinus pyralis sulfotransferase ST3 - pHis8-4

    Depositor

    Jing-Ke Weng

    Article

    Fallon et al Biochemistry. 2016 May 26.

    Insert

    Photinus pyralis sulfotransferase 3
    

    Use

    Tags

    8x-His,TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFGL820

    Plasmid

    #58221

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    ILV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ILV2 native promoter

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJKW 0643

    Plasmid

    #74121

    Purpose

    Photinus pyralis luciferin sulfotransferase LST - pHis8-4

    Depositor

    Jing-Ke Weng

    Article

    Fallon et al Biochemistry. 2016 May 26.

    Insert

    Photinus pyralis firefly luciferin sulfotransferase
    

    Use

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFGL820-2

    Plasmid

    #58222

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    ILV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ILV2 native promoter

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. {Ud}RpL14.Dm

    Plasmid

    #53220

    Purpose

    {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescue

    Depositor

    Diethard Tautz

    Insert

    RpL14 (RpL14 Fly)
    

    Use

    Attb drosophila melanogaster transformation

    Tags

    Expression

    Mutation

    14 synonymous mutations introduced

    Promoter

    endogenous promoter

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK579

    Plasmid

    #72426

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites

    Depositor

    T. J. Kappock

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    4 silent mutations introduce unique internal SpeI…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DinoIII-arrO-N

    Plasmid

    #128143

    Purpose

    Dinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marina

    Depositor

    Senjie Lin

    Article

    Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

    Insert

    Rifampin ADP-ribosylating transferase + potential promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAct1IHPT-4

    Plasmid

    #37844

    Depositor

    Peggy Lemaux

    Article

    Myeong-Je et al Volume 138, Issue 2, 23 November 1998, P

    Insert

    Actin promoter and intron
    

    Use

    Plant transformation

    Tags

    HPT (Hygromycin phosphotransferase) and nos 3…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pLCS99

    Plasmid

    #209327

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of untagged protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-TfR-OVA

    Plasmid

    #64600

    Purpose

    Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Transmembrane domain of Transferrin Receptor (TfR…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge