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We narrowed to 2,581 results for: pCas

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Showing: 281 - 300 of 2581 results

  5. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  10. Hygro-Cas9 donor

    Plasmid
    #86883
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
    Depositor
    Danwei Huangfu
    Article
    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86883-plasmid-map-sequence-id-173280

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  11. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    Collection of MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly.
    Depositor
    Daniel Voytas
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV-RSV-SpCas9 (AAV8)

    Viral Prep
    #85450-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-RSV-SpCas9 (#85450). In addition to the viral particles, you will also receive purified pAAV-RSV-SpCas9 plasmid DNA. RSV-driven expression of SpCas9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hetian Lei
    Article
    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.
    Promoter
    pRSV
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only

  13. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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Showing: 281 - 300 of 2581 results