We narrowed to 986 results for: transfection

1. pCaRNA1&2

   Plasmid

   #86087

   Purpose

   Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA1, RNA2
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

   Plasmid

   #169097

   Purpose

   Cas9 and Citrine expression in transfected cells

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Development. 2021 Mar 9. pii: dev.193565

   Insert

   Cas9-2A-Citrine
   

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry_sgRNA-ver2

   Plasmid

   #126776

   Purpose

   gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   sgRNA (for SpCas9)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry_gRNA

   Plasmid

   #80457

   Purpose

   gRNA expression vector with mCherry transfection control

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Inserts

   sp Cas9 gRNA
   mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 and chicken β-actin promoter

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-ZoAl-RTD-3xF-ORF

   Plasmid

   #238486

   Purpose

   For production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD644/645->AA

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-ZoAl-END-3xF-ORF

   Plasmid

   #238485

   Purpose

   For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD1041/1054->AA

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-ZoAl-ENT-3xF-ORF

   Plasmid

   #238484

   Purpose

   For production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   R1103A

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-TaGu-RTD-3xF-ORF

   Plasmid

   #238490

   Purpose

   For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD660/661->AA

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-TaGu-END-3xF-ORF

   Plasmid

   #238489

   Purpose

   For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD1057/1070->AA

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. eGFP Y93H CC editing reporter

    Plasmid

    #198885

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGC)

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP Y93H AC editing reporter

    Plasmid

    #198883

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGA)

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PAGFP_NS2B3

    Plasmid

    #195755

    Purpose

    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

    Depositor

    Partha Mondal

    Article

    Varghese et al bioRxiv 2022.12.18.520514

    Insert

    Dengue (Type-2) NS2B3
    

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    No mutations

    Promoter

    CMV

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

    Plasmid

    #194925

    Purpose

    mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

    Depositor

    Hao Xu

    Article

    Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    hPGK

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)

    Plasmid

    #184394

    Purpose

    Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC1821_pCR8-SMN2-DN-RG6-SA

    Plasmid

    #118655

    Purpose

    Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    SMN2-DN-RG6-SA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFP Y93H GC editing reporter

    Plasmid

    #198884

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGG)

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-715MRPprom-MRPcucuc

    Plasmid

    #87199

    Purpose

    Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    RMRP (RMRP Human)
    

    Expression

    Mammalian

    Mutation

    GAGAG91-95 to CUCUC

    Promoter

    human RMRP

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xG1 PylT sfGFP 150TAG

    Plasmid

    #154767

    Purpose

    plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPWCh

    Plasmid

    #124238

    Purpose

    Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.

    Depositor

    Peter Vilmos

    Article

    Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    UASp

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TVMV-HRV-3C Proteases-mCherry

    Plasmid

    #243796

    Purpose

    Encodes for mammalian expression of TVMV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

    Depositor

    Cole DeForest

    Article

    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

    Insert

    TVMV and HRV-3C proteases
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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