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We narrowed to 1,438 results for: transfection

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Showing: 281 - 300 of 1438 results

  2. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  3. pcDNA3.1-ME-Flag

    Plasmid
    #101860
    Purpose
    Mds1-Evi-1 flag tagged for transfection into mammalian cells
    Depositor
    Archibald Perkins
    Insert
    Mds1-Evi-1 (Mecom Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101860-plasmid-map-sequence-id-211085

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  4. pCSThaP6ERT(#184)

    Plasmid
    #184103
    Purpose
    cyclofen-inducible mouse Pax6 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    HA-MmPax6-ERT2
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6+T7
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184103-plasmid-map-sequence-id-365942

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  5. pSG1017-2xBsaI-SaKKH-2xUGI_P2A_EGFP

    Plasmid
    #239450
    Purpose
    Codon-optimized S. aureus KKH Cas9 with 2x UGI. 2x BsaI sites for easy cloning of varying deaminases for cytosine base editing. EGFP can serve as a transfection marker.
    Depositor
    Alexis Komor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239450-plasmid-map-sequence-id-476833

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  6. pSG1040-rA1-SaKKH-2xUGI_P2A_EGFP

    Plasmid
    #239463
    Purpose
    Cytosine base editor with codon-optimized S. aureus KKH Cas9, rAPOBEC1 deaminase, and 2x UGI. EGFP can be used as a transfection marker.
    Depositor
    Alexis Komor
    Insert
    S.a. rA1 CBE 2xUGI
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239463-plasmid-map-sequence-id-476830

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  7. pCSKaedenERT(#100)

    Plasmid
    #184064
    Purpose
    cyclofen-inducible Kaede nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Kaede-nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184064-plasmid-map-sequence-id-365948

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  8. pCSTG4FER3(#190)

    Plasmid
    #184069
    Purpose
    cyclofen-inducible GAL4 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Gal4bdFF-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184069-plasmid-map-sequence-id-365692

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  9. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid
    #126478
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or47a (Or47a Fly)
    Orco (Orco Fly)
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available Since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126478-plasmid-map-sequence-id-246565

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  11. pCAG-GluA1-scFV-Halo

    Plasmid
    #230065
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230065-plasmid-map-sequence-id-460116

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  12. pCSmp38ERT(#143)

    Plasmid
    #184077
    Purpose
    cyclofen-inducible P38 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfP38a-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184077-plasmid-map-sequence-id-365706

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  15. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Available Since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92073-plasmid-map-sequence-id-275942

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  20. pAAV-UbC-mCherry Rab5

    Plasmid
    #115239
    Purpose
    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab5a (RAB5A Human, Homo sapiens)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human UbC
    Available Since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115239-plasmid-map-sequence-id-222083

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Showing: 281 - 300 of 1438 results