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pSSa23

Plasmid

#177373

Purpose

Expresses red-shifted luciferase under TEF1 promoter in Candida glabrata

Depositor

Dominique Sanglard

Article

Schrevens et al J Fungi (Basel). 2021 Oct 9;7(10). pii:

Insert

CgLUCopt-mut2

Promoter

TEF1

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSSa23

Plasmid

#177373

Purpose

Expresses red-shifted luciferase under TEF1 promoter in Candida glabrata

Depositor

Dominique Sanglard

Article

Schrevens et al J Fungi (Basel). 2021 Oct 9;7(10). pii:

Insert

CgLUCopt-mut2

Promoter

TEF1

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

Plasmid

#162278

Purpose

Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN1A Variant 1, stabilized (SCN1A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp2946. Silent codon c…

Promoter

CMV

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

Plasmid

#162278

Purpose

Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN1A Variant 1, stabilized (SCN1A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp2946. Silent codon c…

Promoter

CMV

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133398

Plasmid

#152940

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133398

Plasmid

#152940

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SVOP_STOP

Plasmid

#161471

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SVOP (SVOP Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SVOP_STOP

Plasmid

#161471

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SVOP (SVOP Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR1A DHFR.dn-cHSF1

Plasmid

#134726

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Gateway Cloning

Tags

DHFR

Promoter

None

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR1A DHFR.dn-cHSF1

Plasmid

#134726

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Gateway Cloning

Tags

DHFR

Promoter

None

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCL1011

Plasmid

#252432

Purpose

RbAp46 tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

RbAp46 (RBBP7 Human)

Tags

mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL1011

Plasmid

#252432

Purpose

RbAp46 tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

RbAp46 (RBBP7 Human)

Tags

mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM049

Plasmid

#247635

Purpose

to generate mRNA for membrane mStayGold(E138D) for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(E138D)

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM049

Plasmid

#247635

Purpose

to generate mRNA for membrane mStayGold(E138D) for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(E138D)

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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mBaoJin-JIP4

Plasmid

#244978

Purpose

Express JIP4 in mammalian cells with mBaoJin tag (monomeric StayGold)

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

JIP4 (SPAG9 Human)

Tags

mBaoJin

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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mBaoJin-JIP4

Plasmid

#244978

Purpose

Express JIP4 in mammalian cells with mBaoJin tag (monomeric StayGold)

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

JIP4 (SPAG9 Human)

Tags

mBaoJin

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrTagRFP-T-P2A-post-eGRASP

Plasmid

#111590

Purpose

An AAV vector that expresses myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrTagRFP-T-P2A-post-eGRASP

Use

AAV

Promoter

tetO

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrTagRFP-T-P2A-post-eGRASP

Plasmid

#111590

Purpose

An AAV vector that expresses myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrTagRFP-T-P2A-post-eGRASP

Use

AAV

Promoter

tetO

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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MEK1-DD-V5

Plasmid

#217298

Purpose

Lentivirus vector for expression of MEK1 S218D/S222D

Depositor

Channing Der

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

V5

Mutation

S218D/S222D

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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MEK1-DD-V5

Plasmid

#217298

Purpose

Lentivirus vector for expression of MEK1 S218D/S222D

Depositor

Channing Der

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

V5

Mutation

S218D/S222D

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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PB_iETV2_P2A_GFP_Puro

Plasmid

#168805

Purpose

PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ETV2 (ETV2 Synthetic, Human)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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PB_iETV2_P2A_GFP_Puro

Plasmid

#168805

Purpose

PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ETV2 (ETV2 Human, Synthetic)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA3mt

Tags

Myc

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA3mt

Tags

Myc

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-EF1α-H2B-HaloTag-IRES-puro

Plasmid

#247460

Purpose

Piggybac based mammalian expression vector for histone H2B HaloTag, driven by the EF1a promoter.

Depositor

Kazuhiro Maeshima

Article

Hibino et al Nat Commun. 2024 Aug 21;15(1):7152. doi:

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-EF1α-H2B-HaloTag-IRES-puro

Plasmid

#247460

Purpose

Piggybac based mammalian expression vector for histone H2B HaloTag, driven by the EF1a promoter.

Depositor

Kazuhiro Maeshima

Article

Hibino et al Nat Commun. 2024 Aug 21;15(1):7152. doi:

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Alpha 5 integrin-GFP

Plasmid

#15238

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

alpha 5 integrin (ITGA5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Alpha 5 integrin-GFP

Plasmid

#15238

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

alpha 5 integrin (ITGA5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Eco

Plasmid

#35617

Depositor

Arthur Nienhuis, Patrick Salmon

Insert

Ecotropic MLV envelope

Expression

Mammalian

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Eco

Plasmid

#35617

Depositor

Arthur Nienhuis, Patrick Salmon

Insert

Ecotropic MLV envelope

Expression

Mammalian

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge