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ACTN2-pEGFP

Plasmid

#52669

Purpose

Overexpression of GFP-tagged α-actinin-2

Depositor

Johannes Hell

Article

Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

Insert

actinin 2 (ACTN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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reporter-gT1

Plasmid

#47320

Purpose

TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

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tetO-Hand2

Plasmid

#46028

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Hand2 (Hand2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCB168

Plasmid

#37389

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

dynein cytoplasmic 1 intermediate chain 1 (DYNC1I1 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5M-TACE

Plasmid

#31714

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

TACE (ADAM17 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-EPHA2

Plasmid

#23926

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHA2 (EPHA2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMXs-p53DD

Plasmid

#22729

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

Trp53 (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

deleted amino acids 14-301

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 77L

Plasmid

#19308

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Mutation

S77L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl2-Maob

Plasmid

#18001

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Mitochondria-targeted Bcl-2 (BCL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Bcl-2's C terminal replaced with mitochondri…

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
pUC19/human IL-8

Plasmid

#17610

Depositor

Joost Oppenheim

Article

Matsushima et al J Exp Med. 1988 Jun 1. 167(6):1883-93.

Insert

IL8 (CXCL8 Human)

Expression

Bacterial

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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SBE-Luc

Plasmid

#16527

Depositor

Bert Vogelstein

Article

Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

Insert

Smad binding element

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk3a2

Plasmid

#13759

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-14-3-3 zeta GST

Plasmid

#13278

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 zeta (YWHAZ Bovine)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
p4027 GST-ubiquitin

Plasmid

#10861

Depositor

Peter Howley

Article

Scheffner et al Cell. 1993 Nov 5. 75(3):495-505.

Insert

ubiquitin (UBI4 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pCruzHA SIRT1 G261A

Plasmid

#10963

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G261A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR

Plasmid

#171240

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor (AR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-STING_gRNA3

Plasmid

#127640

Purpose

Knock-out of human STING

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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spNW25

Plasmid

#173484

Purpose

Expresses spNW25 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW25

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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