We narrowed to 3,169 results for: EED

1. p-miR-SC

   Plasmid

   #65055

   Purpose

   Expression of miR148a with scrambled seed in mammalian cells

   Depositor

   Cristina Fillat

   Article

   Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

   Insert

   hsa-miR-148a (mutated) (MIR148A Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mutpRL-TK 6xUTR

   Plasmid

   #40761

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   6 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

3. mutpRL-TK 2xUTR

   Plasmid

   #40757

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   2 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

4. mutpRL-TK 4xUTR

   Plasmid

   #40759

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   4 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. mutpRL-TK 5xUTR

   Plasmid

   #40760

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   5 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. mutpRL-TK 1xUTR

   Plasmid

   #40756

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pLuc-CDS-m640-1+2

   Plasmid

   #42106

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Mutation

   Silent mutations were introduced in two seed-comp…

   Promoter

   SV40

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLuc-CDS-m640-2

   Plasmid

   #42105

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Mutation

   Silent mutations were introduced in the seed-comp…

   Promoter

   SV40

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLuc-CDS-m215-1+2

   Plasmid

   #42103

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Mutation

   Silent mutations were introduced in two seed-comp…

   Promoter

   SV40

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLuc-CDS-m640-1

    Plasmid

    #42104

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Silent mutations were introduced in the seed-comp…

    Promoter

    SV40

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLuc-CDS-m215-1

    Plasmid

    #42101

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Silent mutations were introduced in the seed-comp…

    Promoter

    SV40

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. MDH1-miR-196a-2SM-PGK-GFP-Blast

    Plasmid

    #31512

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a2 (Mir196a-2 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pPBT-PE2-PuroTK-pegRNA_GG

    Plasmid

    #173222

    Purpose

    piggyBac transposon containing both PE2 and a pegRNA cloning site.

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    PE2
    PuroTK
    pegRNA cloning cassette
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    CMV, P2A, and hU6

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRES2-EGFP-SHP2WT

    Plasmid

    #12283

    Depositor

    Anton Bennett

    Article

    Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

    Insert

    SHP-2WT (PTPN11 Human)
    

    Tags

    Myc (not canonical: KLISEEDL)

    Expression

    Mammalian

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pG3H-PE3max-attR1R2

    Plasmid

    #213049

    Purpose

    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Inserts

    Cas9 nickase
    M-MLV Reverse Transcriptase
    

    Use

    Note: this plasmid needs helper pvs1-vir2 for rep…

    Expression

    Plant

    Mutation

    H840A, R240K, N413K,

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXW109Hg(RS-RinA-E11)2

    Plasmid

    #123151

    Purpose

    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, PmerT, PhrpLE, PrinA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

    Plasmid

    #175563

    Purpose

    pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    GOXL1
    

    Tags

    Citrine

    Expression

    Plant

    Promoter

    RUBY

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXW117As(RS-E11-RinA)2

    Plasmid

    #123146

    Purpose

    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J117, ParsR, PhrpLE, Pecf11

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe HA-∆Raf1(S642A):ER* puro

    Plasmid

    #72572

    Purpose

    Expresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    Raf1 (RAF1 Human)
    

    Use

    Retroviral

    Tags

    ER* and HA

    Expression

    Mammalian

    Mutation

    amino acids 306-648, serine 642 mutated to alanine

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

20. EGFP-hPCM2w/b delta6

    Plasmid

    #47588

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b delta6 (ATP2B2 Human)
    

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    6 C-terminal AA deleted (needed for PDZ binding)

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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