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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,209 results for: EED

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Showing: 2981 - 3000 of 3209 results

  1. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  2. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Available Since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

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  7. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pMDS1

    Plasmid
    #239460
    Purpose
    For simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusion
    Depositor
    Miguel de Lucas
    Article
    Banda et al Sci. Adv., 11, eadw9153 (2025)
    Type
    Empty backbone
    Tags
    VENUS:3xHA:2A:mTurqN7
    Expression
    Bacterial and Plant
    Available Since
    Oct. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    239460-plasmid-map-sequence-id-488473

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  19. pCS2-MfnG-EcYRS-EGFP*

    Plasmid
    #191119
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Tags
    His tag
    Expression
    Mammalian
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191119-plasmid-map-sequence-id-373722

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Showing: 2981 - 3000 of 3209 results