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pRK5_CKAR3

Plasmid

#238411

Purpose

Mammalian expression of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

CKAR3 (PKC sensor)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mutCKAR3

Plasmid

#238412

Purpose

Mammalian expression of a phosphorylation deficient (T to A) mutant of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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MKT074

Plasmid

#231997

Purpose

MicF RNA toehold switch with NanoLuc complementation peptide reporter

Depositor

Melissa Takahashi

Article

Diaz et al MicroPubl Biol. 2025 Jan 2;2025:10.17912

Insert

MicF RNA toehold switch

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pUlaR

Plasmid

#216327

Purpose

T7 promoter driving expression of ulaR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

L-Ascorbate-6-P-responsive transciptional repressor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAM254

Plasmid

#226898

Purpose

mEOS3.2-IDR*-(7aaSG)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

7aaSG Prescission GSS-FLAG-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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MAP80A_MS2

Plasmid

#220624

Purpose

tRNA reporter vector

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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KNtp-SUR1 core fusion

Plasmid

#216212

Purpose

for expression of KNtp-SUR1 fusion protein in BacMam system

Depositor

Lei Chen

Article

Wang et al Front Pharmacol. 2022 Jun 30;13:929684.

Insert

SUR1 core

Tags

GFP-MBP

Expression

Mammalian

Mutation

deletion of TMD0

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pH2B-6AA-ffDronpa

Plasmid

#214606

Purpose

Expresses reversibly switchable fluorescent protein ffDronpa targeted to the nucleus

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

ffDronpa-H2B

Tags

H2B targetting with a linker of 6AA

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pffDronpa2F-peroxisome

Plasmid

#214608

Purpose

Expresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisome

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

ffDronpa2F-peroxisome

Tags

peroxisome targeting sequence (PTS)

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-neo C-HAonly HPV16 E7 E80A/D81A

Plasmid

#191541

Purpose

Protein expression of HPV16 E7 E80A/D81A

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

HPV16 E7

Use

Retroviral

Tags

HA

Mutation

E80A, D81A

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAFNF-EGFP

Plasmid

#163609

Purpose

Expresses EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Egfp

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-Cv3

Plasmid

#99351

Purpose

Synthetic sequence for single-stranded DNA production

Depositor

Mark Bathe

Article

Veneziano et al Sci Rep. 2018 Apr 25;8(1):6548. doi: 10.

Insert

Line from The Crucible Act 3

Expression

Bacterial

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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Pmo_1188_TNXB/p plate_10

Plasmid

#107643

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

1188_TNXB/p plate_10

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30B

Plasmid

#87082

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30B (Trim30b Mouse)

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-PH(Btk)KD(Akt1)

Plasmid

#86636

Purpose

Expresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

PH(Btk)KD(Akt1)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+8NmCherry-ABCB4a

Plasmid

#34941

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB4a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+8NCerulean-ABCB4a

Plasmid

#34945

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB4a

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+8CCerulean-ABCG2a

Plasmid

#34946

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCG2a

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+NmCitrine-ABCB1a

Plasmid

#34956

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pWJPrP31

Plasmid

#22105

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP exon 3 EcoR1 fragment

Use

Construction of targeting vector

Mutation

genomic EcoR1 fragment containing exon 3 of PrP

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
pHEN2-SG4

Plasmid

#196071

Purpose

Express wild type SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4

Tags

3XFLAG and 6XHis

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET29a-human histone 4-in-One

Plasmid

#223237

Purpose

Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

Depositor

Weixing Zhao

Inserts

His6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII

Tags

Dual StrepII, His6, TEV protease site, and VSVG

Expression

Bacterial

Mutation

A103G

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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NFAT luciferase reporter

Plasmid

#10959

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

IL-2 promoter (IL2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pLS-mP

Plasmid

#81225

Purpose

Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

Type

Empty backbone

Use

Lentiviral

Promoter

Derived from pGL4.23

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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AP1

Plasmid

#179678

Purpose

Accessory plasmids

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[SD8] gIII

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pJSK659

Plasmid

#205128

Purpose

Expression of GFP-SnxA in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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3xERRE-luciferase

Plasmid

#37851

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

3 copies of ERRE upstream of luciferase

Use

Luciferase

Promoter

SV40

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Lck-mStayGold

Plasmid

#234539

Purpose

to label the membrane of cells with a fluorescent protein (mStayGold)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mStayGold(Ando)

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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