We narrowed to 11,554 results for: Vars;

1. pAB094a

   Plasmid

   #79241

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4; PC araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD; PC

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAS12125_PEAR-GFP

   Plasmid

   #177178

   Purpose

   PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2T-CMV-evoCjCas9-TadCDd-BlastR

   Plasmid

   #202558

   Purpose

   Mammalian expression of evoCjCas9 TadCDd

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-TadCDd
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3 mSmoothened WT

   Plasmid

   #126408

   Purpose

   Full length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

   Insert

   m-Smoothened (Smo Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide_CjCas9-Puro

   Plasmid

   #202564

   Purpose

   hU6-BsmBI-gRNA-Puro

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   CjCas9 sgRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p416 25Q GPD

   Plasmid

   #1177

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. LEPG

   Plasmid

   #111160

   Purpose

   miR-E (miR-30 variant)-based RNAi with GFP reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-UGI-nCas9(H840A)-GSVG

   Plasmid

   #187411

   Purpose

   DddA GSVG variant

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA GSVG variant
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p416 103Q GPD

   Plasmid

   #1180

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(-)-mIFNa2

    Plasmid

    #135098

    Purpose

    Protein expression of mouse IFNa2 in mammalian cells

    Depositor

    Pablo Penaloza-MacMaster

    Article

    Palacio et al J Exp Med. 2020 Dec 7;217(12). pii: 1520

    Insert

    mIFN-α2 (Ifna2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid

    #115698

    Purpose

    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    SSKar2-sfpHluorin-HDEL
    

    Use

    Tags

    ER retention signal containing HDEL and ER signal…

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDJSVT86

    Plasmid

    #108965

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

    Type

    Empty backbone

    Use

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CamKIIa-moxBFP-WPRE

    Plasmid

    #194976

    Purpose

    AAV vector to drive expression of moxBFP under the control of CamKIIalpha promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    moxBFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

    Plasmid

    #159853

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mNeon(PATC)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-CMV-evoCjCas9-BE4max-BlastR

    Plasmid

    #194061

    Purpose

    Mammalian expression of evoCjCas9 CBE4max

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-flex-oChIEF-citrine

    Plasmid

    #50973

    Purpose

    flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    inverted and inserted between two lox sites

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p416 72Q GPD

    Plasmid

    #1179

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 72Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-P3-evoCjCas9-TadCDd_gRNA

    Plasmid

    #202563

    Purpose

    P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-TadCDd
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge