We narrowed to 5,806 results for: pcas

1. p44PPV-eGFP

   Plasmid

   #241907

   Purpose

   Part 2 of a two-plasmid PCA biosensor composed of the PcaV repressible PPV promoter upstream from a reporter gene (eGFP) on a second plasmid

   Depositor

   Neil Dixon

   Article

   F M Machado et al J Biol Eng. 2019 Nov 27;13:91. doi: 10.1

   Insert

   His6-eGFP
   

   Use

   Synthetic Biology

   Mutation

   none

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

   Viral Prep

   #73179-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Available Since

   June 14, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pHSP02

   Plasmid

   #117259

   Purpose

   Genome editing for Lactobacillus plantarum WCSF1

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
   

   Use

   Other

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p131CB10-tphKAB

   Plasmid

   #241911

   Purpose

   TPA catabolic operon under the control of a PCA inducible biosensor

   Depositor

   Neil Dixon

   Article

   Chacon et al Trends Biotechnol. 2025 Mar;43(3):647-67

   Insert

   tphK, tphA2, tphA3, tphB, tphA1
   

   Use

   Synthetic Biology

   Mutation

   none

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHSB04X

   Plasmid

   #117258

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
   

   Use

   Other

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pISFba1

   Plasmid

   #226820

   Purpose

   Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

   Depositor

   Sheng Yang

   Article

   Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

   Insert

   ISFba1
   

   Expression

   Bacterial

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pISEre1

   Plasmid

   #226819

   Purpose

   Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISEre1 guide RNA targeting pMB1 ori

   Depositor

   Sheng Yang

   Article

   Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

   Insert

   ISEre1
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCY5h

   Plasmid

   #160295

   Purpose

   Yeast CRISPR plasmid targeting the hphMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCY5n

   Plasmid

   #160296

   Purpose

   Yeast CRISPR plasmid targeting the natMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCY5k

    Plasmid

    #160294

    Purpose

    Yeast CRISPR plasmid targeting the kanMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKB12 (pDEST-DHFR F[3] C-term, HygR)

    Plasmid

    #210486

    Purpose

    pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCY5.1kh

    Plasmid

    #160297

    Purpose

    Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCY5.1nh

    Plasmid

    #160299

    Purpose

    Yeast CRISPR plasmid targeting the natMX and hphMX cassettes

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCY5.1kn

    Plasmid

    #160298

    Purpose

    Yeast CRISPR plasmid targeting the kanMX and natMX cassettes

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDN0606 (pDEST-DHFR F[3] N-term,HygR)

    Plasmid

    #210488

    Purpose

    pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

    Plasmid

    #210485

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

    Plasmid

    #210487

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC45A3

    Plasmid

    #132167

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC45A3 (SLC45A3 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDONR221-SLC45A3_STOP

    Plasmid

    #161333

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC45A3 (SLC45A3 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pgTS40

    Plasmid

    #169630

    Purpose

    pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    U6-driven gRNA expression and PCAG-driven SpCas9 expression
    

    Expression

    Mammalian

    Promoter

    U6 / PCAG

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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