Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 60,007 results for: tra

Clear keywords
Showing: 3021 - 3040 of 60007 results

  1. N295A (EcN) MetNIQ in pBAD

    Plasmid
    #118256
    Purpose
    N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 295 to Alanine (Ala)
    Promoter
    None and araBAD Promotoer
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118256-plasmid-map-sequence-id-228530

    Enlarge

  2. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid
    #118260
    Purpose
    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118260-plasmid-map-sequence-id-228496

    Enlarge

  8. VC-Y705F+K49R-STAT3

    Plasmid
    #203721
    Purpose
    Expresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10
    Insert
    STAT3 tagged with VC (aa 158-238) (STAT3 Human)
    Tags
    VC (aa 158-238) for bimolecular fluorescence comp…
    Expression
    Mammalian
    Mutation
    Y705F+K49R substitutions
    Promoter
    CMV
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203721-plasmid-map-sequence-id-401591

    Enlarge

  9. VC-Y705F+K685R-STAT3

    Plasmid
    #203722
    Purpose
    Expresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10
    Insert
    STAT3 tagged with VC (aa 158-238) (STAT3 Human)
    Tags
    VC (aa 158-238) for bimolecular fluorescence comp…
    Expression
    Mammalian
    Mutation
    Y705F+K685R substitutions
    Promoter
    CMV
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203722-plasmid-map-sequence-id-401596

    Enlarge

  10. pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201821
    Purpose
    An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    SYN1
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201821-plasmid-map-sequence-id-399618

    Enlarge

  11. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201822
    Purpose
    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    CaMKii
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201822-plasmid-map-sequence-id-399617

    Enlarge

  12. mGFP-hNET-A457P

    Plasmid
    #193365
    Purpose
    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells
    Depositor
    Harald Sitte
    Article
    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:
    Insert
    human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
    Tags
    monomeric GFP (mGFP)
    Expression
    Mammalian
    Mutation
    A457P (GCC to CCC)
    Promoter
    CMV
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193365-plasmid-map-sequence-id-381451

    Enlarge

  13. mGFP-hNET-R121A/K334A/R440A

    Plasmid
    #193366
    Purpose
    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells
    Depositor
    Harald Sitte
    Article
    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:
    Insert
    human Norepinephrine Transporter (SLC6A2 Human)
    Tags
    monomeric GFP (mGFP)
    Expression
    Mammalian
    Mutation
    R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …
    Promoter
    CMV
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193366-plasmid-map-sequence-id-381450

    Enlarge

  16. N295A (EcN), M163A (EcI) MetNIQ in pBAD

    Plasmid
    #118261
    Purpose
    N295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M163A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118261-plasmid-map-sequence-id-228528

    Enlarge

  17. N295A (EcN), M107A (EcI) MetNIQ in pBAD

    Plasmid
    #118259
    Purpose
    N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M107A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118259-plasmid-map-sequence-id-228500

    Enlarge

  18. N295A (EcN), F103A (EcI) MetNIQ in pBAD

    Plasmid
    #118258
    Purpose
    N295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (F103A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118258-plasmid-map-sequence-id-228498

    Enlarge

  19. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral Prep
    #104492-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pGP-AAV-syn-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep
    #104487-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 3021 - 3040 of 60007 results