We narrowed to 2,346 results for: puc19

1. pSLQ5135: pCAG-BFP-GuideArray

   Plasmid

   #210606

   Purpose

   Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   pCAG-BFP-GuideArray
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDM098

   Plasmid

   #216818

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold
   

   Use

   Tags

   FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJAID5XY

   Plasmid

   #165051

   Purpose

   Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pILGFP95

   Plasmid

   #83550

   Purpose

   Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PDA1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PDA1

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pShoHELIX-sgRNA_entry (BO3)

   Plasmid

   #181784

   Purpose

   Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTX1-SMN1-exon7-ESE1

   Plasmid

   #126041

   Purpose

   In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

   Depositor

   Frédéric Allain

   Article

   Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

   Insert

   SMN1 exon7 ESE1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTetQCas-8+IS186

   Plasmid

   #170636

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEFS-ccdB-12X MS2_mPGK-Puro

   Plasmid

   #177809

   Purpose

   Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

   Type

   Empty backbone

   Use

   Tags

   12X MS2 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pILGFPH7

   Plasmid

   #83554

   Purpose

   Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   ADH2 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH2

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP97

    Plasmid

    #83551

    Purpose

    Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CYC1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge