We narrowed to 3,355 results for: actin

Showing: 3061 - 3080 of 3355 results

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-SVIP-Strep-HA

Plasmid

#113491

Purpose

Expression of human SVIP with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

SVIP (SVIP Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN(deltaNLS)-pmGFP

Plasmid

#26867

Purpose

Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pclbw-PAGFPomp25

Plasmid

#58427

Purpose

Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

paGFP-omp25 (Synj2bp Mouse)

Use

Retroviral

Tags

PAGFP

Expression

Mammalian

Mutation

contains aa's 109-145 of mouse omp25

Promoter

cmv-bactin

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGal4AD-CIB1

Plasmid

#28245

Purpose

Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

Gal4AD (CIB1, GAL4 Budding Yeast)

Tags

CIB1

Expression

Yeast

Promoter

ADH1

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pBJ FLAG-hPar1

Plasmid

#53226

Purpose

mammalian expression of human Par1 with N-terminal FLAG tag

Depositor

Shaun Coughlin

Article

Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

Insert

Par1 (F2R Human)

Tags

FLAG and bovine prolactin signal sequence (MDSKGS…

Expression

Mammalian

Mutation

Deletion of aa1-39 (native signal peptide)

Promoter

SV40

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag JIP3b

Plasmid

#53458

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-EGxxFP-Cetn1

Plasmid

#50717

Purpose

Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

Cetn1 (Cetn1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 JIP3a

Plasmid

#53457

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

T7

Expression

Mammalian

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only
CibN

Plasmid

#209671

Purpose

Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

Depositor

Sanjeevi Sivasankar

Article

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Split-TurboID-N and pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-GIT1

Plasmid

#15226

Depositor

Rick Horwitz

Article

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

L425V, E476K and L583P

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D][S227D]-FLAG

Plasmid

#199324

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Aspartic Acid

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-S100A10

Plasmid

#107200

Purpose

Expresses YFP-human S100A10 fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

Insert

S100A10 (S100A10 Human)

Tags

Yellow Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP3

Plasmid

#75165

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIP3

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIP3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-VCPIP1

Plasmid

#85671

Purpose

Expression of VCPIP1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

VCPIP1 (VCPIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag Jip1b

Plasmid

#52123

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-CIP4

Plasmid

#178364

Purpose

Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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GIT1A-FLAG

Plasmid

#15225

Depositor

Rick Horwitz

Article

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1 sgTollip

Plasmid

#196546

Purpose

Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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