We narrowed to 4,339 results for: Pet2;

1. pET28a-His6-ODC-Ctag

   Plasmid

   #163614

   Purpose

   Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

   Depositor

   Mark Howarth

   Article

   Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

   Insert

   ODC (ODC1 Human)
   

   Tags

   C-tag and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21b-MBPx-MBPx-SpyTag003

   Plasmid

   #216291

   Purpose

   Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   MBPx-MBPx-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-T7-NlaCas3-NLS-6xHis

   Plasmid

   #180214

   Purpose

   Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   NlaCas3c
   

   Tags

   6xHis and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SARS-CoV-2 NSP13

   Plasmid

   #176492

   Purpose

   Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

   Depositor

   Xiaodong Cheng

   Article

   White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

   Insert

   SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   I570 from SARS-CoV NSP13 was mutated to V to conv…

   Promoter

   T7

   Available Since

   Jan. 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET22-Nsp10-TEV-6His

   Plasmid

   #154957

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
   

   Tags

   TEV-6His

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET28-MHL: nsp16_SARS-CoV-2

   Plasmid

   #167850

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp16

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp16
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28a-SENP7 (catalytic domain)

   Plasmid

   #16360

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP7 catalytic domain (SENP7 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 640 to 984 catalytic domain of human S…

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-UBC9-K14R-A129K

   Plasmid

   #228948

   Purpose

   express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   changed Lysine 14 to Arginine, changed Alanine 12…

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-2xMreBN-mCherry-His

   Plasmid

   #133626

   Purpose

   Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   2xMreB-mCherry-His
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-MBP-CBX1-eReader

    Plasmid

    #135089

    Purpose

    Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered
    

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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