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pWZL Neo Myr Flag CDK5

Plasmid

#20449

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK5 (CDK5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CLK3

Plasmid

#20465

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CLK3 (CLK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag FASTK

Plasmid

#20485

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

FASTK (FASTK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCas3-Km

Plasmid

#207999

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSR348

Plasmid

#124713

Purpose

Expression of narX under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narX (narX )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S193* - LacI is truncated and likely deactivated.…

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMIG-Flag-RIPK1-wt

Plasmid

#102677

Purpose

retroviral expression plasmid for RIPK1-wt

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Promoter

unknown

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
KHBD00343

Plasmid

#34466

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11992 (Rel Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
p3E-GFP no-pA

Plasmid

#80813

Purpose

GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP no pA

Expression

Mammalian

Promoter

GFP

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-UPRT

Plasmid

#69310

Purpose

expresses UPRT cDNA

Depositor

Chris Doe

Article

Gay et al Nat Protoc. 2014 Feb;9(2):410-20. doi: 1

Insert

UPRT cDNA

Tags

HA

Expression

Bacterial

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
pAM1706

Plasmid

#40242

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

PpsbAI

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Plasmid

#159798

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Plasmid

#159799

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSABAD505

Plasmid

#45615

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Tags

GFP_C and GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAPKAP1

Plasmid

#20528

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CDK7

Plasmid

#20452

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK7 (CDK7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pME-mKate2 no stop

Plasmid

#80806

Purpose

red fluorophore without stop codon

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-mKate2 no stop

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMB41

Plasmid

#73087

Purpose

N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

Avi-GFP tag

Tags

Avi and GFP

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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Mito-SNAP

Plasmid

#206480

Purpose

Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)

Tags

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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