We narrowed to 32,171 results for: uros

1. pCLJ-F2P-LoxP-PGK-PuroR-LoxP

   Plasmid

   #227502

   Purpose

   Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   Prdm8 promoter
   

   Use

   AAV and Cre/Lox

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti spCas9 T2A mNeonGreen P2A puro

   Plasmid

   #122183

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   mNeonGreen
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-Pan-Neurofascin (extracellular) [A12/18R]

   Plasmid

   #177438

   Purpose

   Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. delta DE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52381

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Expression

   Mammalian

   Mutation

   deleted -3194 - -1768 upstream from OCT4 ATG

   Promoter

   OCT4

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-HF1RA-P2A-GFP-PGK-Puro

   Plasmid

   #110866

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-AU1

   Plasmid

   #162102

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-FLAG

   Plasmid

   #162104

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   VSVg-ProtC-FLAG

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-FLAG

   Plasmid

   #162110

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-FLAG

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-AU1

    Plasmid

    #162085

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEpicVector3G_Puro-T2A-EGFP-VSVg-FLAG-AU1

    Plasmid

    #162088

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-FLAG-AU1

    Plasmid

    #162094

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-FLAG

    Plasmid

    #162081

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-StrepTagII-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

    Plasmid

    #134865

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Membrin (GOSR2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-CAG-REV1-p2A-GFP-PuroR

    Plasmid

    #153001

    Purpose

    Overexpression of human REV1

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    REV1 (REV1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1-TfR20-SNAP-IRES-Puro

    Plasmid

    #171018

    Purpose

    Lentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    FitzGerald et al Nat Commun. 2020 Sep 8;11(1):4482. doi:

    Insert

    Transferrin receptor (Human) - SNAP-tag (TFRC Human)
    

    Use

    Lentiviral

    Tags

    SNAPtag and TfR

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-VQR-P2A-GFP-PGK-Puro

    Plasmid

    #110861

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

    Plasmid

    #177320

    Purpose

    Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

    Depositor

    Xiaolin Nan, Steven Chu

    Article

    Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Insert

    KRAS 4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP-derived dimerization domain (DD, or DmrB) an…

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    TetOn-CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PLKO-mcherry-luc-puro-miR-211

    Plasmid

    #29784

    Depositor

    Carl Novina

    Article

    Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

    Insert

    miR-211
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid

    #202571

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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