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FL-fibulin-3 pcDNA4

Plasmid

#29700

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pEG BacMam C term 3C eGFP myc

Plasmid

#160685

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk3a2

Plasmid

#15748

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172504

Purpose

Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1202

Plasmid

#44484

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, MCS

Use

Multiple cloning site vector

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS4

Plasmid

#218713

Purpose

Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS4 (BBS4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

I354T (t1110c)

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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GST-BRD (1079-1198)

Plasmid

#99507

Purpose

Bacterial expression of mouse CBP bromodomain

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

mouse CBP BRD (1079-1198) (Crebbp Mouse)

Tags

GST

Expression

Bacterial

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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NELFE-mOrange pHis-//

Plasmid

#53303

Purpose

Expresses His-tagged NELF-E-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

NELF-E protein (Nelf-E Fly)

Tags

His and mOrange

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk2b1

Plasmid

#13756

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b1 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hEFA6A

Plasmid

#206412

Purpose

Mammalian expression of hEFA6A

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

Insert

EFA6A (PSD Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pconst #1-RFP

Plasmid

#81075

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23119

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-FB-E25TAG-F6C

Plasmid

#176546

Purpose

Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

IgG affinity protein

Tags

His tag

Expression

Bacterial

Mutation

change phenylalanine 6 to cysteine; change glutam…

Promoter

T5

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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MTK3a_007

Plasmid

#123760

Purpose

Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PTS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRGECO1

Plasmid

#125247

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Tags

NES signal

Expression

Mammalian

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1001

Plasmid

#44476

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, [4-3] Gateway

Use

Gateway destination vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pOET3-N-TEV-Twin-Strep

Plasmid

#203323

Purpose

Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site (ENLYFQG) and Twin-strep-tag

Expression

Insect

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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