We narrowed to 9,807 results for: CAG

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pCAG-GFP1 POD-nAb-WPRE

Plasmid

#244973

Purpose

Expresses a fusion protein of a nanobody against GFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

GFP1 POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pT/CAGGS-NRASG12V/D38A-IRES-mVenus

Plasmid

#236077

Purpose

Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Inserts

mVenus
NRAS G12V D38A (NRAS Human)

Use

Sleeping beauty transposon plasmid

Mutation

G12V D38A

Promoter

CAGGS

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PDGFRb559-562del-IRES2-EGFP

Plasmid

#204355

Purpose

Expresses a mutant PDGFRb gene (559-562 deletion).

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Mutation

559-562 deletion

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ULK1 (836-1050)-MBP

Plasmid

#203562

Purpose

Mammalian expression of ULK1 (836-1050) with MBP tag

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ULK1 (ULK1 Human)

Tags

MBP-10His

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194723

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

Plasmid

#161923

Purpose

To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting HSD17B11 exon 1

Use

CRISPR

Promoter

U6

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2d-cs-TEV-STREP

Plasmid

#178912

Purpose

Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d

Tags

C term strep and N term mcherry

Expression

Mammalian

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-loxp-stop-loxp-IL17RA-QY-tTA-T2A-IL17RC-TEV-IRES-TagBFP-PA

Plasmid

#255643

Purpose

Express mouse IL-17A CyCLoPs cassette followed by TagBFP as a marker

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Inserts

IL-17RA (Il17ra Mouse)
IL-17RC (Il17rc Mouse)
TagBFP

Tags

TEV and tTA

Expression

Mammalian

Promoter

CAG

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#87560

Purpose

Expresses aa397-423 of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa397-423

Use

Cre/Lox and Synthetic Biology

Tags

PYL

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#87559

Purpose

Expresses aa1-396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa1-396

Use

Cre/Lox and Synthetic Biology

Tags

ABI

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPH-T2A-GFP-shP53

Plasmid

#102895

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPH-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI (JDW 1494)

Plasmid

#242592

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-CAGEc-ABL1 (Npu)

Plasmid

#241420

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product BCR-ABL, Figure 3, Extended Figure 6-7 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

CAGEc-ABL1 (Npu) (ABL1 Synthetic, Human)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-BCRn-CAGEn (Npu)

Plasmid

#241419

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product BCR-ABL, Figure 3, Extended Figure 6-7 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

BCRn-CAGEn (Npu) (BCR Synthetic, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-EGFP-ATG13

Plasmid

#223797

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 (ATG13 Human)

Tags

GST-TEV-EGFP

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro 3XFLAG-hATXN7L3

Plasmid

#225725

Purpose

Transfection of ATXN7L3 with 3XFLAG tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

ATXN7L3 (ATXN7L3 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X Y381H

Plasmid

#225723

Purpose

Transfection of USP27X (Y381H) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y381H (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

Y381H

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-S1pr1-R120A

Plasmid

#203707

Purpose

Overexpression of FLAG-S1PR1-R120A mutant.

Depositor

Timothy Hla

Insert

Flag-S1PR1-R120A (S1PR1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R120A

Available Since

Dec. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-TSF

Plasmid

#203545

Purpose

FIP200 NTD-TwinStrep-Flag mammalian expression

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Tags

TwinStrep Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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