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pCfB8786

Plasmid

#126920

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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EC70133

Plasmid

#209507

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70134

Plasmid

#209508

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRS425ADS

Plasmid

#20119

Depositor

Jay Keasling

Article

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Insert

amorphadiene synthase

Use

Synthetic Biology

Expression

Yeast

Available Since

March 2, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-Ace2_D92N_E199V-mNeon-ST

Plasmid

#129261

Purpose

Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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CDC42

Plasmid

#55693

Purpose

expression of mouse CDC42

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

CDC42

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
mt TEFM

Plasmid

#205058

Purpose

TEFM protein expression

Depositor

Smita Patel

Article

Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

Insert

TEFM (TEFM Human)

Expression

Bacterial

Mutation

Codon optimized

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1

Plasmid

#187367

Purpose

Expresses human Plekhh1 labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-GNMT

Plasmid

#166177

Purpose

Gateway entry vector for N. furzeri GNMT

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

GNMT

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mNeon-Zeo

Plasmid

#207731

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Puro

Plasmid

#207724

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-mNeon

Plasmid

#207698

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-V5

Plasmid

#207697

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A22

Plasmid

#166210

Purpose

Gateway entry vector for N. furzeri SLC25A22

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SLC25A22

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5-p110α-WT

Plasmid

#55709

Purpose

eukaryotic expression of Flag tagged p110a WT

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha

Tags

flag

Expression

Mammalian

Promoter

T7

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-p23

Plasmid

#108224

Purpose

Mammalian expression vector for mCherry fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

mCherry-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLN379 (FuGW-S(E2F1)p-mKate2)

Plasmid

#105177

Purpose

synthetic promoter E2F1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-STX6

Plasmid

#31581

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

syntaxin 6 (STX6 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pSfi-HspLacZ

Plasmid

#33351

Depositor

David Kingsley, Doug Mortlock

Article

Mortlock et al Genome Res. 2003 Sep;13(9):2069-81. Epub

Insert

Hsp68 promoter (Hspa1a Mouse)

Use

Mouse Targeting

Tags

LacZ

Expression

Mammalian

Promoter

Hsp68 (Hspa1a)

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only
pT2-Tbait-UAS-Positron-ST

Plasmid

#129266

Purpose

UAS-driven expression of Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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