We narrowed to 9,000 results for: ef1;

1. TFORF0089

   Plasmid

   #142532

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-Regnase-3 WT-T2A-copGFP

   Plasmid

   #222660

   Purpose

   Lentiviral vector to express mouse Regnase-3 WT

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12c (Zc3h12c Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mutated stop codon prior to a T2A peptide

   Promoter

   EF1α

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWR63(YUM1)

   Plasmid

   #203336

   Purpose

   episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   YUM1-targeting sequence
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWR63

   Plasmid

   #203331

   Purpose

   episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRHDGAL43C

   Plasmid

   #218279

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRHDGAL3C

   Plasmid

   #218276

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCh-665

   Plasmid

   #92254

   Purpose

   Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   EF1A

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3` CC: Hygro – GFP-N – loxP – mScarlet

   Plasmid

   #219564

   Purpose

   3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   3` CC: Hygro – GFP-N – loxP – mScarlet
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF2-FL-hMer_gR1

   Plasmid

   #225993

   Purpose

   Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor

   Depositor

   Raymond Birge, Sergei Kotenko

   Article

   Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

   Insert

   human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF2-FL-hTyro_gR1

    Plasmid

    #225995

    Purpose

    Expresses a chimeric contruct having N-terminal human Tyro3 and C-terminal human Interferon Gamma Receptor

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

    Insert

    human Tyro3 N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (TYRO3, IFNGR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF2-FL-hAxl_gR1

    Plasmid

    #225994

    Purpose

    Expresses a chimeric contruct having N-terminal human Axl and C-terminal human Interferon Gamma Receptor

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

    Insert

    human Axl N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (AXL, IFNGR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF2-EFGR-hAxl

    Plasmid

    #225997

    Purpose

    Expresses a chimeric contruct having N-terminal human EGFR and C-terminal human Axl

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

    Insert

    human EGFR N-Terminal human Axl C-Terminal Fusion (EGFR, AXL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLP-PIGA (pLP140)

    Plasmid

    #168461

    Purpose

    Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

    Depositor

    Jef Boeke, Matthew Maurano

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cre-IRES-PuroR

    Plasmid

    #30205

    Purpose

    Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

    Depositor

    Darrell Kotton

    Article

    Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

    Insert

    Cre recombinase (cre Enterobacteria phage P1)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiMPH v2 (Neo)

    Plasmid

    #92065

    Purpose

    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Insert

    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid

    #229498

    Purpose

    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    MYC 5'Untranslated region + destabilized GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PEmax_AAVS1_knockin_HDR_donor

    Plasmid

    #223000

    Purpose

    HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PEmax
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-puro-N-3HA-SREBP1-C-Flag

    Plasmid

    #134286

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    EF1a

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF-LEF and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, Human, S. pyogenes)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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