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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,039 results for: pET

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Showing: 3161 - 3180 of 10039 results

  1. pKK-FLAG-BirA-TEV

    Plasmid
    #105770
    Purpose
    Expression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    FLAG-BirA-TEV
    Expression
    Mammalian
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    105770-plasmid-map-sequence-id-203337

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  8. HUWE1

    Plasmid
    #25211
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    huwe1.4005.4374 (HUWE1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    HECT domain
    Available since
    Feb. 18, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  11. ITCH

    Plasmid
    #36197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH (ITCH Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains HECT domain
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  13. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

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  15. PET01-TAF5-ex8-Minigene-SRSF1 motif WT

    Plasmid
    #218972
    Purpose
    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    Expression
    Mammalian
    Promoter
    RSV LTR
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218972-plasmid-map-sequence-id-436110

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  18. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131300-plasmid-map-sequence-id-254651

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  19. pTCF158 Human beta-2 microglobulin

    Plasmid
    #180457
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Human beta-2 microglobulin (B2M Human)
    Expression
    Bacterial
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180457-plasmid-map-sequence-id-355801

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Showing: 3161 - 3180 of 10039 results