We narrowed to 15,451 results for: pET

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His-SUMO-flag-hDDI2_pET11a

Plasmid

#169121

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV-AICD

Plasmid

#69927

Purpose

bacterial expression of MBP-TEV-AICD

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

47 aa long

Promoter

T7

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a-ClbB-NRPS-NHis

Plasmid

#49217

Purpose

NRPS module of ClbB containing CAT domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbB-NRPS

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa4-1080 of reference sequence NP_754362…

Promoter

T7

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CAT-L713F

Plasmid

#173944

Purpose

Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET-KIX (586-672)

Plasmid

#99336

Purpose

Bacterial expression of mouse CBP KIX domain

Depositor

Peter Wright

Article

Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

Insert

CBP KIX (586-672) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-HIS-ybbr

Plasmid

#101238

Purpose

SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

SdrG_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-TGFα-GSY-SPM

Plasmid

#163615

Purpose

Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

TGFa-GSY-SPM (TGFA Human, Synthetic)

Tags

C-tag and His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/L28F

Plasmid

#118584

Purpose

Bacterial expression of E.coli DHFR N23PP and L28F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

L28 mutated to F28, N23 mutated to P23, a proline…

Promoter

T7

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(D130N)-RagC

Plasmid

#99642

Purpose

Bacterial expression of RagA(D130N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

D130N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-6His-SUMO-Nsp14

Plasmid

#154956

Purpose

Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

Depositor

Justin Kollman

Insert

6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)

Tags

6His-Thrombin-SUMO

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-SENP5 (catalytic domain)

Plasmid

#16358

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP5 catalytic domain (SENP5 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 567 to 755 catalytic domain of human S…

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only
PET28a-cpLumiLuc-Rapamycin sensor

Plasmid

#186292

Purpose

Expression Rapamycin sensor in e coli

Depositor

Kirill Alexandrov

Article

Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

Insert

cpLumiLuc-Rapamycin sensor

Tags

HisTag

Expression

Bacterial

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(T21N)-RagC

Plasmid

#99644

Purpose

Bacterial expression of RagA(T21N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

T21N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET24a HAB1 (172-511)

Plasmid

#177844

Purpose

Bacterial Expression of HAB1

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

Protein phosphatase 2C 16

Tags

6xHis-GST

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-G97 (for GFP)

Plasmid

#130715

Purpose

Plasmid encoding the bacterial expression of LAMA-G97 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-G97

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-K14R

Plasmid

#185759

Purpose

Bacterial expression of His6-Ubc9-K14R

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

K14R

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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PET-B-eSpCas9-NLS-6xHis

Plasmid

#207383

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, amino acids 1005-1013 repl…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-MBP-TEV-PWWP_DNMT3A

Plasmid

#186970

Purpose

Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

DNMT3A PWWP domain (DNMT3A Human, Synthetic)

Tags

His-MBP

Expression

Bacterial

Mutation

PWWP domain (residues 278–427)

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-YPet-WPRE

Plasmid

#193342

Purpose

AAV-mediated expression of YPet under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

YPet

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB2-eGFP-6xHis

Plasmid

#130982

Purpose

Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB2-GFP-6xHis (Mapre2 Mouse)

Tags

eGFP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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