Addgene

We narrowed to 2,374 results for: Alb

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pET23-CBD-(-PP)-EGFP

Plasmid

#12597

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

Cdc42 Biosensor (WAS Human)

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

F271C

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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CTM-PC1

Plasmid

#41573

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

FLM (4192-4303)

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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NTSP-PC1

Plasmid

#41575

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

Myc/Myc/His

Expression

Mammalian

Mutation

4106-4218 of FLS PC1

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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CTSP-PC1

Plasmid

#41577

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

2xMyc/His and myc/myc/his

Expression

Mammalian

Mutation

FLS (4172-4303)

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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NTM (FLM-PC1Δ137)

Plasmid

#41572

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

sequence of PKD1 FLM from 4078 - 4273

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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FLM-PC1Δ30

Plasmid

#41568

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Δ30; sequence of PKD1 from 4078 - 4273

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1Δ45

Plasmid

#41569

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Δ45; sequence of PKD1 from 4078 - 4258

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1Δ67

Plasmid

#41570

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Δ67; sequence of PKD1 from 4078 - 4236

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1Δ103

Plasmid

#41571

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Δ103; sequence of PKD1 from 4078 - 4200

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
BSA2_His

Plasmid

#202767

Purpose

bovine serum albumin design BSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA2

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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BSA3_His

Plasmid

#202768

Purpose

bovine serum albumin design BSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T3_gRNA

Plasmid

#199335

Purpose

pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type III sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b-pknG

Plasmid

#102814

Purpose

Expression of PknG in E.coli

Depositor

Jean Pieters

Article

Walburger et al Science. 2004 Jun 18;304(5678):1800-4. E

Insert

pknG

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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68Q-ymsfGFP

Plasmid

#128560

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymsfGFP

Use

Tags

ymsfGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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mpeg:lamp2-mCherry-stop; cmlc2:GFP

Plasmid

#213740

Purpose

Expresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

lamp2-mCherry

Use

Zebrafish

Tags

Expression

Mutation

Promoter

mpeg1.1

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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mpeg:Rab5c-stop-polyA; cmlc2:GFP

Plasmid

#213737

Purpose

Expresses mCherry-Rab5c in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

mCherry-Rab5c

Use

Zebrafish

Tags

mCherry

Expression

Mutation

Promoter

mpeg1.1

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDA010.188

Plasmid

#206792

Purpose

J23113.dCas9

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

J23113

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCK389

Plasmid

#206791

Purpose

Sp.dCas9, pTet.MCP-SoxS, J23119.gRNA

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Inserts

dCas9
MCP-SoxS
scRNA

Use

Tags

Expression

Mutation

Promoter

J23119 and Tet

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBT009.119.DA9

Plasmid

#206803

Purpose

Expresses DA9 scRNA for activation of pDA303

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

DA9 scRNA

Use

Tags

Expression

Mutation

Promoter

J23119

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T2_gRNA

Plasmid

#197647

Purpose

pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type II sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
zCREST3:Cas9green; T1_gRNA

Plasmid

#199332

Purpose

pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type I sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
25Q-ymsfGFP

Plasmid

#128559

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

25Q-Httex1 (without proline-rich region) fused to ymsfGFP

Use

Tags

ymsfGFP

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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68Q-ymScarlet

Plasmid

#128562

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymSarlet

Use

Tags

ymScarlet

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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25Q-ymScarlet

Plasmid

#128561

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

25Q-Httex1 (without proline-rich region) fused to ymSarlet

Use

Tags

ymScarlet

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAD06 K. lactis telomerase RNA hairpin

Plasmid

#61895

Purpose

Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin (nt 229-273)

Use

Tags

Expression

Mutation

Promoter

T7

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pSTAR-mOrange2-sfGFP

Plasmid

#115693

Purpose

Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

Depositor

David Suter

Article

Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

Insert

SA-mOrange2-sfGFP-SD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PV-NES-GFP

Plasmid

#17301

Depositor

Anton Bennett

Article

Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

Insert

PV-NES-GFP (Pvalb Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-EGFP-Cdc42(wt)

Plasmid

#12599

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (WT) (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a-4SS-HEWL

Plasmid

#39233

Depositor

Harald Schwalbe

Article

Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

Insert

Hen egg white lysozyme (LYZ Chicken)

Use

Tags

Expression

Bacterial

Mutation

N-terminal Met

Promoter

T7

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MCP/PCP RNA Lantern

Plasmid

#226095

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEFmEGFP-mDia2

Plasmid

#25407

Depositor

Arthur Alberts

Article

Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Insert

mDia2 (Diaph3 Mouse)

Use

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Encodes mDia2

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pADH100

Plasmid

#90980

Purpose

NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-UBC-EGFP-2A-BoNT-B (LC)

Plasmid

#185722

Purpose

Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

botulinum neurotoxin type B light chain

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GG-EBNA-OCT4

Plasmid

#69537

Purpose

Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

5 concatenated gRNA transcriptional cassettes targeting OCT4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-mDia1FH2deltaN

Plasmid

#25419

Depositor

Arthur Alberts

Article

Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

Insert

diaph1 (Diaph1 Mouse)

Use

Tags

YFP A206K

Expression

Mammalian

Mutation

mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

Promoter

Available since

July 20, 2010

Availability

Academic Institutions and Nonprofits only
pRRG389-PC2

Plasmid

#99087

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea prohormone convertase 2 (PC2)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

prohormone convertase 2 (PC2)

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRG757-smedwi-1

Plasmid

#99074

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Smedwi-1

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Smedwi-1 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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