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We narrowed to 4,399 results for: ARA-2

Showing: 301 - 320 of 4399 results

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RCH1:GA2ox2-GFP

Plasmid

#108567

Purpose

Arabidopsis transformation, expresses GA2ox2 with RCH1 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

GA2ox2 (GA2OX2 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

RCH1

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGA2ox2:GUS

Plasmid

#108564

Purpose

Arabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

GA2ox2 promoter (GA2OX2 Mustard Weed)

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

GA2ox2

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL

Plasmid

#200120

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Venus-Bcl2-pEGFP-C1

Plasmid

#177417

Purpose

To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL(C145A)

Plasmid

#200121

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Use

Tags

Myc

Expression

Mammalian

Mutation

C145A

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

Plasmid

#184335

Purpose

Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

Nrxn3-alpha-GPI (Nrxn3 Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHDT2:GUS

Plasmid

#108444

Purpose

Arabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 promoter (HD2B Mustard Weed)

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

HDT2

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT2:HDT2-GFP

Plasmid

#108566

Purpose

Arabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 (HD2B Mustard Weed)

Use

Tags

GFP

Expression

Plant

Mutation

Promoter

HDT2

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTracer-EF-mFxh

Plasmid

#21927

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2006 Feb . 26(4):1209-22.

Insert

Fxh (Rbfox2 Mouse)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only
RCH1:HDT1,HDT2-RNAi

Plasmid

#108446

Purpose

Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoter

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Inserts

HDT1 (HDA3 Mustard Weed)
HDT2 (HD2B Mustard Weed)

Use

RNAi

Tags

Expression

Plant

Mutation

Promoter

RCH1

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only
pET21b-RBD-JA1

Plasmid

#167126

Purpose

Expresses SARS-CoV-2 RBD in bacterial system

Depositor

David Margulies

Article

Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

Insert

SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)

Use

Tags

Expression

Bacterial

Mutation

residues (333 to 529 aa)

Promoter

T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pfMDH_N191A

Plasmid

#67724

Purpose

Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed asparagine 191 to alanine

Promoter

T7

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pfMDH_N300D

Plasmid

#67728

Purpose

Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed asparagine 300 to aspartate

Promoter

T7

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pfMDH_N300S

Plasmid

#67729

Purpose

Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed asparagine 300 to serine

Promoter

T7

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

Plasmid

#187344

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

KRAB WSR7EEE

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Plasmid

#195540

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_FL

Plasmid

#157867

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltalinker

Plasmid

#157870

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the linker

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaliner

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaCTD

Plasmid

#157871

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaCTD

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaNTD

Plasmid

#157869

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaNTD

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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