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Plasmid#68218PurposeModule composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expressionDepositorInsertLuc / Renilla / P19
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnos / 35S / 35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-Full-length
Plasmid#194550PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)-Strep
Plasmid#164444PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to GlycinePromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)
Plasmid#160618PurposeModule for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).DepositorInsertTnos:NptII:Pnos-SF-P35s:hCas9:tNos
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterPnos, 35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-HMGB1-MUT-Patchless
Plasmid#194563PurposeDox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant "Patchless" (K184Rfs*44, "Patchless" variant) Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44, truncation by stop codon after Lys209PromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-SAP25-126-186(pL91)
Plasmid#124387PurposeE.Coli produced SAP25 SID purified by FPLC nickel columnDepositorInsertGB1-His-SAP25-126-186 (SAP25 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911(pL91)
Plasmid#124390PurposeE.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) T2C, C90A, T198C
Plasmid#220235Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationT2C, C90A, T198CPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C, T259C
Plasmid#220234Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172C, T259CPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D-AtUBI10-NbGP41-sfGFP-VP64-GB1
Plasmid#202012PurposeMoclo level 1 - Module D, Promoter: AtUBI10, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: pea rbcsE9DepositorInsertNbGP41-sfGFP-VP64-GB1
UseSynthetic Biology; Moclo level 1 vectorTagsGB1 and sfGFPExpressionMutationPromoterAtUBI10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)
Plasmid#199242PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS191C, I225A, L228A, W231APromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-Tev-ALIX-V (348-702)
Plasmid#189819PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).DepositorInsertGB1-His-Tev-ALIX-V (348-702) (PDCD6IP Human)
UseTagsGB1-His-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-HsHelz_1475-1642del1595-1609-GB1His6_AH
Plasmid#148889PurposeBacterial Expression of HsHelz_1475-1642-del1595-1609DepositorInsertHsHelz_1475-1642-del1595-1609 (HELZ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-HsHelz_1643-1792del1746-1764-GB1His6_AH
Plasmid#148892PurposeBacterial Expression of HsHelz_1643-1792-del1746-1764DepositorInsertHsHelz_1643-1792-del1746-1764 (HELZ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-HsHelz_1793-1942del1890-1907-GB1His6_AH
Plasmid#148895PurposeBacterial Expression of HsHelz_1793-1942-del1890-1907DepositorInsertHsHelz_1793-1942-del1890-1907 (HELZ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-ScEap1p_107-122-Y107IA108KS110T-GB1_AG
Plasmid#148813PurposeBacterial Expression of ScEap1p_107-122-Y107IA108KS110TDepositorInsertScEap1p_107-122-Y107IA108KS110T (EAP1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmRoquin_702-819-ILLVtoEEEE-GB1His6_AE
Plasmid#148625PurposeBacterial Expression of DmRoquin_702-819-ILLVtoEEEEDepositorInsertDmRoquin_702-819-ILLVtoEEEE (roq Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmRoquin_702-819-L805EV809E-GB1His6_AE
Plasmid#148626PurposeBacterial Expression of DmRoquin_702-819-L805EV809EDepositorInsertDmRoquin_702-819-L805EV809E (roq Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-CteIF4G_996-1033-YLLtoAAA-GB1_AF
Plasmid#148706PurposeBacterial Expression of CteIF4G_966-1033-YLLtoAAADepositorInsertCteIF4G_966-1033-YLLtoAAA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmRoquin_501-819-GB1-His6_AD
Plasmid#148534PurposeBacterial Expression of DmRoquin_501-819DepositorInsertDmRoquin_501-819 (roq Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only