We narrowed to 10,138 results for: SEC

1. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227904

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUSE-URA3-Sec7-mEYFP

   Plasmid

   #218968

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

   Depositor

   Benjamin Glick

   Insert

   SEC7-mEYFP (SEC7 Budding Yeast)
   

   Use

   Gene replacement

   Tags

   mEYFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUSE-URA-Sec7-mGold

   Plasmid

   #218967

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

   Depositor

   Benjamin Glick

   Insert

   SEC7-mGold (SEC7 Budding Yeast)
   

   Use

   Gene replacement vector for yeast

   Tags

   mGold

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUSE-URA3-Sec7-msYFP2

   Plasmid

   #218969

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2

   Depositor

   Benjamin Glick

   Insert

   SEC7-msYFP2 (SEC7 Budding Yeast)
   

   Use

   Tags

   msYFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SEC7-2EGFP-ter-NAT

   Plasmid

   #184764

   Purpose

   Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   SEC7 (SEC7 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SEC7-2Katushka-ter-Hygromycin

   Plasmid

   #184772

   Purpose

   Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   SEC7 (SEC7 Budding Yeast)
   

   Use

   Tags

   2Katushka

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS405 SEC66-VAC8-GFP

   Plasmid

   #166840

   Purpose

   Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, SEC66 Budding Yeast)
   

   Use

   Tags

   GFP and SEC66 transmembrane domain

   Expression

   Yeast

   Mutation

   SEC66 transmembrane domain

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV306-ribo-Sec-APEX2

   Plasmid

   #111701

   Purpose

   Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (integrating)

   Depositor

   Jessica Seeliger

   Article

   Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

   Insert

   APEX2
   

   Use

   Tags

   V5 and mpt63 signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   hsp60-ribo

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEW-Venus-Sec16A-deltaN

   Plasmid

   #66605

   Purpose

   Lentiviral vector for express truncated form of Sec16A as a fusion with Venus FP

   Depositor

   David Stephens

   Article

   Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

   Insert

   Sec16A (SEC16A Human)
   

   Use

   Tags

   Venus (EYFP derivative)

   Expression

   Mammalian

   Mutation

   encodes amino acids 1107-1217 of human Sec16A

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. FLAG-Sec31a S1163A #13#904

    Plasmid

    #42118

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    Sec31a (SEC31A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    1163A

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

11. FLAG-Sec31a W985A #790

    Plasmid

    #42122

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    Sec31a (SEC31A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    G742R and W985A NOTE: the mutation is actually W…

    Promoter

    CMV

    Available since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

12. FLAG-Sec31a-4SA #33#630

    Plasmid

    #42114

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    Sec31a (SEC31A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    527A/779A/1163A/1165A (4SA)

    Promoter

    CMV

    Available since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

13. FLAG-Sec31a-4SD #36#631

    Plasmid

    #42115

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    Sec31a (SEC31A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    527D/779D/1163D/1165D (4SD)

    Promoter

    CMV

    Available since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pC020 - LseCas13a from Listeria seeligeri

    Plasmid

    #91910

    Purpose

    Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LseCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. actb2-SecAnxaV-mKate2-acry-EGFP

    Plasmid

    #123628

    Purpose

    Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    AnnexinA5
    

    Use

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3/flag-pbira(1-g78)-sec61b

    Plasmid

    #154455

    Purpose

    expresses flag-pbira(1-g78)-sec61b/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pbira(1-g78)-sec61b (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-AcGFP-Sec61-IRES-Blast

    Plasmid

    #154009

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    acGFP-Sec61 (SEC61B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid

    #131312

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMS4 (Empty insertion vector with SEC)

    Plasmid

    #154837

    Purpose

    Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    SEC
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

    Plasmid

    #141025

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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