We narrowed to 13,275 results for: crispr cas9 plasmids

1. PtPuc3_diaCas9_sgRNA

   Plasmid

   #109219

   Purpose

   Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

   Depositor

   Per Winge

   Article

   Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

   Inserts

   LHCF2 promoter
   diaCas9
   LHCF1 terminator
   U6 promoter
   sgRNA
   U6 3' region
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   LHCF1 terminator, LHCF2 promoter, U6 3' regi…

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_dCas9-2xAM

   Plasmid

   #92220

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA to be inserted into Bbs I sites
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

   Plasmid

   #159908

   Purpose

   Mutagenesis of Htr2a

   Depositor

   Larry Zweifel

   Insert

   Htr2a (Htr2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROPseq-EFS-SpCas9-P2A-blast

   Plasmid

   #106477

   Purpose

   single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_dCas9-2xAM

   Plasmid

   #92220

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA to be inserted into Bbs I sites
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-dCas9-dDNMT3a-C706S-Hygro

   Plasmid

   #104412

   Purpose

   transient expression of dCas9-dDNMT3a fusion protein

   Depositor

   Stephan Beck

   Insert

   dDNMT3a
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   C706S

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-dCas9-SET7-H297G-Hygro

   Plasmid

   #104415

   Purpose

   transient expression of dCas9-dSET7 fusion protein

   Depositor

   Stephan Beck

   Insert

   dSET7
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   H297G

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUPD2 nickase D10A Cas9 (GB1690)

   Plasmid

   #160587

   Purpose

   Nickase D10A Cas9 for Nt and Ct fusion.

   Depositor

   Diego Orzaez

   Insert

   nickase D10A Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUPD2 nickase D10A Cas9 (GB1691)

   Plasmid

   #160588

   Purpose

   Nickase D10A Cas9 for Nt fusion.

   Depositor

   Diego Orzaez

   Insert

   nickase D10A Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. dSaCas9-KRAB

    Plasmid

    #205250

    Purpose

    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

    Depositor

    Rasmus Bak

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pActin5c-CD-dCas9m4-UDI-attB

    Plasmid

    #104879

    Purpose

    To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9

    Depositor

    Christopher Potter

    Insert

    CD-dCas9(m4)-UDI
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid

    #126881

    Purpose

    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid

    #126882

    Purpose

    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. dSaCas9-KRAB

    Plasmid

    #205250

    Purpose

    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

    Depositor

    Rasmus Bak

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid

    #160587

    Purpose

    Nickase D10A Cas9 for Nt and Ct fusion.

    Depositor

    Diego Orzaez

    Insert

    nickase D10A Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUPD2 nickase D10A Cas9 (GB1691)

    Plasmid

    #160588

    Purpose

    Nickase D10A Cas9 for Nt fusion.

    Depositor

    Diego Orzaez

    Insert

    nickase D10A Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)

    Plasmid

    #205417

    Purpose

    Mutagenesis of Ntsr1, second guide

    Depositor

    Larry Zweifel

    Insert

    Ntsr1 (Ntsr1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9v2

    Plasmid

    #103140

    Purpose

    TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Removed scaffold gRNA from original plasmid

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pActin5c-CD-dCas9m4-UDI-attB

    Plasmid

    #104879

    Purpose

    To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9

    Depositor

    Christopher Potter

    Insert

    CD-dCas9(m4)-UDI
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge