We narrowed to 7,301 results for: poly

1. KG901: pMVP (L3-L2) HA epitope tag + polyA

   Plasmid

   #121749

   Purpose

   pMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. attB-GFP-Dnmt3b6-Poly(A)-NeoR

   Plasmid

   #65552

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   isoform 6

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Poliovirus 1 PV1 isolate 09_05_19 3C protease

   Plasmid

   #228647

   Purpose

   Bacterial expression of the Polio 3C protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Polio virus 3C protease
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pspCas9-POLI-ST2-com vector

   Plasmid

   #176234

   Purpose

   Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

   Plasmid

   #174025

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   eGFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Y40N

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

   Plasmid

   #192552

   Purpose

   Expresses nuclear localized tdTomato under CAG promoter

   Depositor

   Guoping Feng

   Has Service

   AAV PHP.eB and AAV9

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   deleted E273 and G274 compared to FPbase ID: PGG5S

   Promoter

   CAG

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Use

   Tags

   HindIII site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZH23 Dual G-less containing ySSA1 polyA

   Plasmid

   #231647

   Purpose

   yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   SSA1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZH20 Dual G-less containing yCYC1 polyA

   Plasmid

   #231646

   Purpose

   yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   CYC1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-EGFP barcode-1-SV40 polyA

    Plasmid

    #190865

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CAG-EGFP barcode-0-SV40 polyA

    Plasmid

    #190864

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-TgKI-MCS-eGFP-MCS-polyA

    Plasmid

    #174024

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-EGFP barcode-3-SV40 polyA

    Plasmid

    #190867

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-EGFP barcode-4-SV40 polyA

    Plasmid

    #190868

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode4
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-EGFP barcode-2-SV40 polyA

    Plasmid

    #190866

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

    Plasmid

    #232935

    Purpose

    Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    T2A-eGFP-SV40pA with Grb10 homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

    Plasmid

    #218868

    Purpose

    Mammalian expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

    Plasmid

    #84604

    Purpose

    Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

    Depositor

    James Chen

    Article

    Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

    Insert

    mCherry
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    8xGliBS-delta-crystallin minimal promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-RHO-IntC-dCas9C-VPR-polyA

    Plasmid

    #166692

    Purpose

    Express C-terminal part of split dCas9-VPR

    Depositor

    Stylianos Michalakis

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Insert

    IntC-dCas9C-VPR
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge