We narrowed to 1,255 results for: post

1. pRS426-TUB1-internal6xHis (1-440)

   Plasmid

   #60391

   Purpose

   Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4), amino a…

   Promoter

   GAL

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRS424-TUB2 - human TUBB6 Cterminal tail

   Plasmid

   #60403

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. LSM044-2 CMV-TO-CCR7-V2-ADAR2dd(E488Q, T501A) (FLP-IN)

   Plasmid

   #233396

   Purpose

   CCR7-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   CCR7-ADAR2dd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   CMV

   Available Since

   June 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

   Plasmid

   #233443

   Purpose

   PiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available Since

   June 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP

   Plasmid

   #233446

   Purpose

   PiggyBac donor plasmid to overexpress ADAR2dd-PCP

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2dd-PCP-T2A-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

   Plasmid

   #233442

   Purpose

   PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP

   Plasmid

   #233441

   Purpose

   PiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP

   Plasmid

   #233444

   Purpose

   PiggyBac donor plasmid to overexpress ADAR2dd-MCP

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2dd-MCP-T2A-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

   Plasmid

   #233413

   Purpose

   LIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   LIDARSensor(mCherry->Casp9-2A-GFP)-hygro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. XZ054 (PB)SFFV-LIDARsensor (hygR)

    Plasmid

    #233419

    Purpose

    PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integration

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xMS2))cSG-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2)

    Plasmid

    #233430

    Purpose

    LIDAR all components on single transcript (as shown in Figure 4A), using EMCV IRES to separate each component

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    sensor-IRESv0-LIDARst(IRESv0-AVPR2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. XZ098 SFFV-mCherry-LIDARsensor(W)-hIgG1-TM

    Plasmid

    #233429

    Purpose

    Positive control LIDAR reporter RNA (UAG stop codon replaced with UGG) with a membrane-bound human IgG as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-LIDARsensor(W)-hIgG1-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP

    Plasmid

    #233408

    Purpose

    LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xPP7))cSG-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

    Plasmid

    #233415

    Purpose

    LIDAR reporter RNA; secreted NanoLuc Luciferase as marker, SEAP as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. XZ400 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-TEVP (equivalent to EK0461)

    Plasmid

    #233414

    Purpose

    LIDAR reporter RNA; mCherry as marker, TEVP as output (equivalent to EK0461)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xMS2))cSG-TEVP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LSM053 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP (FLP-IN)

    Plasmid

    #233409

    Purpose

    LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, mTagBFP as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN)

    Plasmid

    #233439

    Purpose

    SFFV driven LIDAR reporter with 2 tandem substrate stem loops

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. APL0038 Ac5-mCherry-SGc(stem-1(3xMS2))cSG-EGFP

    Plasmid

    #233410

    Purpose

    AC5 promoter driven LIDAR reporter RNA for drosophila cell expression (mCherry as marker, EGFP as output)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xMS2))cSG-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Ac5

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A)

    Plasmid

    #233378

    Purpose

    PGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in between

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    FKBP-ADAR2dd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    PGK

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. XZ030 CMV-TO-AVPR2-ADAR2(DD-E488Q-T501A) (FLP-IN)

    Plasmid

    #233380

    Purpose

    AVPR2-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and extra V2 tail

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    AVPR2-ADAR2dd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge