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pCDNAIIIB cdc42 QL

Plasmid

#61636

Purpose

mammalian expression of cdc42 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Cdc42 (CDC42 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (kinase dead)

Plasmid

#29544

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
LB124 (Bcl-2 from -1287 to -3934)

Plasmid

#15240

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -3934 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pBS ColI alpha2

Plasmid

#19207

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 type I collagen (COL1A2 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

Plasmid

#26568

Depositor

Diane Lipscombe

Article

Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

contains exons a10, 18a, 31a, 37b, and 46. Del…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pPBpuro-ErbB4

Plasmid

#197363

Purpose

Encoding human ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1_LIM

Plasmid

#109780

Purpose

Protein expression and purification of LASP1_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LASP1_LIM (LASP1 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse eIF2C2

Plasmid

#50354

Purpose

Mouse eIF2C2 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C2 (Ago2 Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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LB335 (Bcl-2 from ATG to -1281)

Plasmid

#15382

Depositor

Linda Boxer

Article

Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from ATG to -1281 with P2

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-MSP1D1deltaH4/2H5

Plasmid

#71715

Purpose

Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMX GFP cdc42 Q61L

Plasmid

#14568

Depositor

Joan Brugge

Article

Hall et al Immunity. 2006 Mar . 24(3):305-16.

Insert

cdc42 Q61L (CDC42 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pCIneo-NHA-Ago2

Plasmid

#115361

Purpose

Expression vector to produce NHA-tagged human Ago2 protein

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

Argonaute 2 (AGO2 Human)

Tags

N peptide and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6CNW)RS

Plasmid

#222873

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_ALDOA_Glycolytic

Plasmid

#109854

Purpose

Protein expression and purification of ALDOA_Glycolytic

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ALDOA_Glycolytic (ALDOA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFE-hCA

Plasmid

#132999

Purpose

pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

hCAII (CA2 Human)

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

Plasmid

#26571

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 31a Contains e…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pWP1_mHAL

Plasmid

#102318

Purpose

expression of mouse cDNA of HAL refractory to the guide in plasmid 4

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Hal (Hal Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV FLIPi P2G_Thy1.1 p53

Plasmid

#19748

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_60.F2

Plasmid

#60208

Purpose

Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)

Tags

FRAP and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 2021-2112 of FRAP with a T2098L mutat…

Promoter

CMV

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-ErbB4CTF

Plasmid

#17803

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

HA

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only