We narrowed to 29,591 results for: PLE

1. pEMS2026

   Plasmid

   #49133

   Purpose

   AAV plasmid with TNNT1-B (Ple301) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple301-iCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TNNT1-B

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pRS406-PHO5-GFP-CERT PH domain EE mutation

   Plasmid

   #58833

   Purpose

   Expresses GFP-Tagged and mutated CERT PH domain in yeast

   Depositor

   Tim Levine

   Article

   Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

   Insert

   collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   changed Arginine 43 to Glutamate

   Promoter

   PHO5

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-EGFP-C-IFT46

   Plasmid

   #218724

   Purpose

   Expresses N-terminally EGFP-tagged IFT46 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT46 (IFT46 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-IFT25

   Plasmid

   #218721

   Purpose

   Expresses N-terminally EGFP-tagged IFT25 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT25 (HSPB11 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

   Plasmid

   #217509

   Purpose

   KD Recombinase dependent conditional 1X ALFA cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
   

   Use

   Tags

   ALFA tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. NSP4-Δ220-234-Flag

   Plasmid

   #210341

   Purpose

   Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Δ220-234

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NSP4-H120N/F121L-Flag

   Plasmid

   #210340

   Purpose

   Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H120N/F121L

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. NSP4-FR-2Strep

   Plasmid

   #210316

   Purpose

   Expresses SARS-CoV-2 NSP4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. NSP4-mCherry

   Plasmid

   #210319

   Purpose

   Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. STIM1-2Strep

    Plasmid

    #210361

    Purpose

    Expresses STIM1 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP10-2Strep

    Plasmid

    #210357

    Purpose

    Expresses FKBP10 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    FKBP10 (FKBP10 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. DNAJC11-2Strep

    Plasmid

    #210354

    Purpose

    Expresses DNAJC11 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    DNAJC11 (DNAJC11 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EC2_2_dCas9_VP64_sgRNA

    Plasmid

    #163707

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VP64+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-sgPLXNB2

    Plasmid

    #86151

    Purpose

    Cas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5FRTTO-NHASt-codoptUL33

    Plasmid

    #135103

    Purpose

    Codon-optimized HSV-1 UL33 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL33 codon optimized
    

    Use

    Tags

    HA-Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRTTO-codoptUL50-CStHA

    Plasmid

    #135105

    Purpose

    Codon-optimized HSV-1 UL50 with C-terminal StrepHA tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL50 codon optimized
    

    Use

    Tags

    Strep-Strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5FRTTO-codoptUL33-CStHA

    Plasmid

    #135106

    Purpose

    Codon-optimized HSV-1 UL33 with C-terminal StrepHA tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL33 codon optimized
    

    Use

    Tags

    Strep-Strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5FRTTO-codoptUS2-CStHA

    Plasmid

    #135107

    Purpose

    Codon-optimized HSV-1 US2 with C-terminal StrepHA tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 US2 codon optimized
    

    Use

    Tags

    Strep-Strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS2046

    Plasmid

    #79654

    Purpose

    ssAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple257-EmGFP WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    PAX6 Enhancer

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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