We narrowed to 7,663 results for: Ski

1. pSLQ10871

   Plasmid

   #183962

   Purpose

   U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   dCas12a(wildtype)
   poly-crRNA array targeting Sox2-Klf4-Oct4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG and U6

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGDP4 tet(A)

   Plasmid

   #112887

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   tet(A)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence was codon optimized for E. coli K12 usin…

   Promoter

   Plac

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIN40C.SFFV.Luciferase.IRES.dTomato

   Plasmid

   #169307

   Purpose

   Constitutive overexpression of Luciferase cDNA with dTomato as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   IRES-dTomato

   Promoter

   SFFV

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172503

   Purpose

   Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGDP3 rmtB

   Plasmid

   #112880

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   rmtB (RmtB Escherichia coli)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGDP4 ermC

   Plasmid

   #112884

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   ermC (ermM Staphylococcus epidermis)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGDP3 cat

   Plasmid

   #112888

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   cat (Brevibacillus brevis) (cat Brevibacillus brevis)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. rs214-minor

   Plasmid

   #113380

   Purpose

   evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   rs2149943 minor allele
   

   Use

   Luciferase

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGDP3 aph(3'')-Ia

   Plasmid

   #112879

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDF0352 HvsCas7-11 with C-term Nes mammalian expression

    Plasmid

    #172516

    Purpose

    Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    C-term Nes HvsCas7-11 mammalian expression
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET151-GyrB

    Plasmid

    #216233

    Purpose

    S. aureus USA300 GyrB Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ10333

    Plasmid

    #183955

    Purpose

    CAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    Nuclease-dead Lb Cas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SiC-V1

    Plasmid

    #133041

    Purpose

    SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    dTomato

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUPER-retro-puro-tetO-CTR1-shRNA

    Plasmid

    #53180

    Purpose

    Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    CTR1 (SLC31A1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

15. MAC3-EGFR

    Plasmid

    #222610

    Purpose

    Expression of MAC3-tagged EGFR

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFR (EGFR Human)
    

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. GAL FLAG103QDProGFPp303

    Plasmid

    #15587

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

    Plasmid

    #193823

    Purpose

    CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    [4xCox8]-ChemoG-NAD
    

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC-SARS-CoV-2 NSP3

    Plasmid

    #158354

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP3 (ORF1ab )
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRevTRE-hygro-GFP-Crumbs3a WT

    Plasmid

    #195217

    Purpose

    Retroviral Tet response vector containing GFP tagged Crumbs3a

    Depositor

    Ben Margolis, David Bryant

    Article

    Schluter et al Mol Biol Cell. 2009 Nov;20(22):4652-63.

    Insert

    CRB3A (CRB3 Human)
    

    Use

    Retroviral

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEY41

    Plasmid

    #191045

    Purpose

    dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    dat-1 (dat-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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