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pccGFP-MalE-pos-2H07

Plasmid

#239088

Purpose

Positive control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of FND10

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 184 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2E11

Plasmid

#239084

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of LRIG3

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 827 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2F12

Plasmid

#239085

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of LRIG3

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 815 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-mCherry-SopF

Plasmid

#229745

Purpose

Expresses mCherry-tagged SopF in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

mCherry-SopF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGH1364

Plasmid

#223681

Purpose

AP1_RE-miniCMV-GFP-pEF-G418R

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Insert

GFP and G418 Resistance

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGH1110

Plasmid

#223682

Purpose

U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperior.retro.neo GFP shScramble

Plasmid

#220357

Purpose

Retroviral expression vector for an shScramble

Depositor

David Kashatus

Article

Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

Insert

shScramble

Use

RNAi and Retroviral

Promoter

H1

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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G1095C_BattB(GA)_jGCaMP7c_IRES_shBleR-P2A-HygroR

Plasmid

#200638

Purpose

jGCaMP7c recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

jGCaMP7c

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-mNeonC

Plasmid

#178461

Purpose

The mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28a

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen

Tags

6xHis

Expression

Bacterial

Mutation

Free cysteine at the C-terminus

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeon

Plasmid

#178462

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTatABmNeonC

Plasmid

#178464

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatBmNeon, TatC

Tags

mNeonGreen fused to TatB

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTatABCmNeon

Plasmid

#178465

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatB, TatCmNeon

Tags

mNeonGreen fused to TatC

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTorD-H6

Plasmid

#169039

Purpose

Escherichia coli TorD with C-terminal 6xHis in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

TorD-H6

Tags

6xHis

Expression

Bacterial

Mutation

The mutation N at position 46 was changed back to…

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NK88

Plasmid

#176612

Purpose

To manipulate ligand spacing.

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

DNA-CARy- aTM (aa291-404)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-MBP_D

Plasmid

#146120

Purpose

Insect Expression of MBP

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

MBP

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pClodAcytCh-GFP-P4M

Plasmid

#113346

Purpose

Bacterial expression of Cherry and GFP-SidM-P4M domain (2 copies)

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

Cherry
GFP-SidM-P4M domain (2 copies of P4M in tandem)

Expression

Bacterial

Promoter

ProD and proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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YCe1760 HC_Kan_IRES2_p8b

Plasmid

#100673

Purpose

part designed to occupy position 8a of EMMA. Functional category: IRES sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

IRES2

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1762 HC_Kan_SV40p_p14

Plasmid

#100674

Purpose

part designed to occupy position 14 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

SV40 promoter

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1883 HC_Kan_p2A_p8

Plasmid

#100675

Purpose

part designed to occupy position 8a of EMMA. Functional category: p2A sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

P2A

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1889 HC_Kan_SV40-polyA_p11

Plasmid

#100678

Purpose

part designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

SV40-polyA

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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